Aswathy Ajayakumar, Vineetha Pookalavan Karicherry, Kandathil Vishal, Jose Jiya, Bhat Sarita G, Manoj Narayanapillai
Department of Applied Chemistry and Interuniversity Center for Nanomaterials and Devices, Cochin University of Science and Technology, Kochi-22, Kerala, India.
Department of Biotechnology, Cochin University of Science and Technology, Kochi-22, Kerala, India.
J Fluoresc. 2024 Jul;34(4):1671-1682. doi: 10.1007/s10895-023-03390-1. Epub 2023 Aug 18.
A simple, efficient, and reversible fluorescent sensor probe, PBA (2,6-dimethyl pyrone barbituric acid conjugate), comprised of a pro-aromatic donor conjugated with a barbituric acid, was developed for the detection of highly toxic mercuric ions. The probe showed high selectivity and "Turn-On" fluorescence response towards Hg among various metal cations such as Na, Mg, Ca, Mn, Fe, Co, Ni, Cu, Zn, Cd, Ba, Hg, and Pb, in both homogeneous and microheterogeneous micelle medium sodium dodecyl sulphate (SDS). The binding stoichiometry, limit of detection (LOD), and binding constant for the PBA-Hg complex were determined. The mechanism of binding was ascertained using the N,N'-dimethylbarbituric acid conjugate of 2,6-dimethylpyran (PDMBA), where no binding interaction by deprotonation is possible. In the presence of cysteamine hydrochloride and trifluoroacetic acid (TFA), the complexation of Hg with PBA was demonstrated to be reversible, indicating its potential for the development of reusable sensors. Moreover, the practical applicability of PBA in monitoring Hg in living cells was also evaluated.
一种简单、高效且可逆的荧光传感器探针PBA（2,6 - 二甲基吡喃巴比妥酸共轭物）被开发用于检测剧毒汞离子，它由一个与巴比妥酸共轭的前芳香供体组成。在十二烷基硫酸钠（SDS）的均相和微非均相胶束介质中，该探针在各种金属阳离子（如Na、Mg、Ca、Mn、Fe、Co、Ni、Cu、Zn、Cd、Ba、Hg和Pb）中对Hg表现出高选择性和“开启”荧光响应。测定了PBA - Hg配合物的结合化学计量比、检测限（LOD）和结合常数。使用2,6 - 二甲基吡喃的N,N'-二甲基巴比妥酸共轭物（PDMBA）确定了结合机制，在该共轭物中不可能通过去质子化发生结合相互作用。在盐酸半胱胺和三氟乙酸（TFA）存在下，Hg与PBA的络合被证明是可逆的，这表明其在开发可重复使用传感器方面的潜力。此外，还评估了PBA在监测活细胞中Hg的实际适用性。
