Bioengineering Program, University of Puerto Rico, Mayagüez, Puerto Rico.
Chemistry Department, University of Puerto Rico, Mayagüez, Puerto Rico.
Pharm Res. 2023 Dec;40(12):2859-2871. doi: 10.1007/s11095-023-03584-z. Epub 2023 Aug 18.
This study describes the first efforts to build a spectral library to identify four cell culture media in powder form with spectra obtained with a handheld Raman spectrometer. These complex mixtures contain over 30 components and are among the most widely used cell culture media.
A total of 32 spectra were collected for the four Dulbecco's Modified Eagle Medium cell culture media and pure materials (glucose and L-glutamine) in powder form. The spectra were preprocessed using standard normal variate with second derivative, and the barcode method before performing principal component analysis (PCA).
The PCA model differentiated the pure glucose and the cell culture media according to the glucose concentration along the first principal component. The second principal component differentiated the three cell culture media with high glucose content according to the pyruvate concentration. The correlation coefficient showed that powdered cell culture media with high glucose concentration have a higher correlation with pure glucose, when compared with the cell culture media with low glucose.
The Raman spectra made it possible to differentiate the four DMEM in the cell culture media from the majority of the external samples used in the method evaluation. However, sample heterogeneity affected the predictions. Additional studies are needed to improve the method's ability to differentiate the DMEM with high glucose.
本研究首次尝试建立一个光谱库，以使用手持式拉曼光谱仪获得的光谱来识别四种粉末状细胞培养基。这些复杂的混合物包含超过 30 种成分，是最广泛使用的细胞培养基之一。
共采集了 32 种粉末形式的四种 Dulbecco's Modified Eagle Medium 细胞培养基和纯物质（葡萄糖和 L-谷氨酰胺）的光谱。在进行主成分分析（PCA）之前，使用标准正态变量和二阶导数对光谱进行预处理，并使用条码法进行预处理。
PCA 模型根据第一主成分上的葡萄糖浓度区分了纯葡萄糖和细胞培养基。第二主成分根据丙酮酸浓度区分了三种高葡萄糖含量的细胞培养基。相关系数表明，与低葡萄糖浓度的细胞培养基相比，高葡萄糖浓度的粉末细胞培养基与纯葡萄糖具有更高的相关性。
拉曼光谱能够区分细胞培养基中的四种 DMEM 与方法评估中使用的大多数外部样本。然而，样品异质性影响了预测。需要进一步的研究来提高该方法区分高葡萄糖 DMEM 的能力。
