Mater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, QLD, Australia.
Blood. 2023 Oct 19;142(16):1339-1347. doi: 10.1182/blood.2023020597.
In this spotlight, we review technical issues that compromise single-cell analysis of tissue macrophages, including limited and unrepresentative yields, fragmentation and generation of remnants, and activation during tissue disaggregation. These issues may lead to a misleading definition of subpopulations of macrophages and the expression of macrophage-specific transcripts by unrelated cells. Recognition of the technical limitations of single-cell approaches is required in order to map the full spectrum of tissue-resident macrophage heterogeneity and assess its biological significance.
在本次焦点讨论中，我们回顾了影响组织巨噬细胞单细胞分析的技术问题，包括产量有限且不具代表性、组织解离过程中的碎片化和残余物产生以及细胞激活等问题。这些问题可能导致对巨噬细胞亚群的定义产生误导，以及非相关细胞表达巨噬细胞特异性转录本。为了绘制组织驻留巨噬细胞异质性的全貌并评估其生物学意义，需要认识到单细胞分析方法的技术局限性。
