Department of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Rev Bras Ginecol Obstet. 2023 Jul;45(7):e393-e400. doi: 10.1055/s-0043-1772186. Epub 2023 Aug 18.
Endometriosis causes a decrease in oocyte quality. However, this mechanism is not fully understood. The present study aimed to analyze the effect of endometriosis on cumulus cell adenosine triphosphate ATP level, the number of mitochondria, and the oocyte maturity level.
A true experimental study with a post-test only control group design on experimental animals. Thirty-two mice were divided into control and endometriosis groups. Cumulus oocyte complex (COC) was obtained from all groups. Adenosine triphosphate level on cumulus cells was examined using the Elisa technique, the number of mitochondria was evaluated with a confocal laser scanning microscope and the oocyte maturity level was evaluated with an inverted microscope.
The ATP level of cumulus cells and the number of mitochondria in the endometriosis group increased significantly ( < 0.05; < 0.05) while the oocyte maturity level was significantly lower ( < 0.05). There was a significant relationship between ATP level of cumulus cells and the number of mitochondrial oocyte ( < 0.01). There was no significant relationship between cumulus cell ATP level and the number of mitochondrial oocytes with oocyte maturity level ( > 0.01; > 0.01). The ROC curve showed that the number of mitochondrial oocytes (AUC = 0.672) tended to be more accurate than cumulus cell ATP level (AUC = 0.656) in determining the oocyte maturity level.
In endometriosis model mice, the ATP level of cumulus cells and the number of mitochondrial oocytes increased while the oocyte maturity level decreased. There was a correlation between the increase in ATP level of cumulus cells and an increase in the number of mitochondrial oocytes.
子宫内膜异位症会导致卵母细胞质量下降。然而，其具体机制尚不完全清楚。本研究旨在分析子宫内膜异位症对卵丘细胞三磷酸腺苷（ATP）水平、线粒体数量和卵母细胞成熟度的影响。
采用实验动物的真实实验性研究，仅有后测对照组设计。将 32 只小鼠分为对照组和子宫内膜异位症组。从所有组中获取卵丘卵母细胞复合物（COC）。使用 ELISA 技术检测卵丘细胞的 ATP 水平，使用共聚焦激光扫描显微镜评估线粒体数量，使用倒置显微镜评估卵母细胞成熟度。
与对照组相比，子宫内膜异位症组的卵丘细胞 ATP 水平和线粒体数量显著增加（<0.05；<0.05），而卵母细胞成熟度显著降低（<0.05）。卵丘细胞 ATP 水平与卵母细胞线粒体数量之间存在显著关系（<0.01）。卵丘细胞 ATP 水平与卵母细胞线粒体数量与卵母细胞成熟度之间不存在显著关系（>0.01；>0.01）。ROC 曲线显示，线粒体卵母细胞数量（AUC=0.672）比卵丘细胞 ATP 水平（AUC=0.656）更能准确地确定卵母细胞成熟度。
在子宫内膜异位症模型小鼠中，卵丘细胞的 ATP 水平和线粒体卵母细胞的数量增加，而卵母细胞成熟度降低。卵丘细胞 ATP 水平的增加与线粒体卵母细胞数量的增加之间存在相关性。
