School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China.
School of Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Tsinghua University, Beijing 100084, China.
J Am Chem Soc. 2023 Aug 30;145(34):18968-18976. doi: 10.1021/jacs.3c06102. Epub 2023 Aug 19.
We report the development of a self-renewable tag (srTAG) for protein fluorescence imaging. srTAG leverages the "on-protein" fluorophore equilibrium between the fluorescent zwitterion and non-fluorescent spirocyclic form and the reversible fluorescence labeling to enable self-recovery of fluorescence after photobleaching. This small-sized srTAG allows 2-6 times longer imaging duration compared to other commonly used self-labeling tags and is compatible with fluorophores with different spectral properties. This study provides a new strategy for fine tuning of self-labeling tags.
我们报告了一种用于蛋白质荧光成像的自更新标签（srTAG）的开发。srTAG 利用“在蛋白质上”荧光体在荧光两性离子和非荧光螺环形式之间的平衡以及可逆荧光标记，使荧光在光漂白后能够自我恢复。与其他常用的自标记标签相比，这种小型 srTAG 允许成像持续时间延长 2-6 倍，并且与具有不同光谱特性的荧光团兼容。这项研究为自标记标签的微调提供了一种新策略。
