Liu Xuezhong, Zhang Lei
Department of Gastrointestinal Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.
Department of General Surgery, Liaocheng Dongchangfu People's Hospital, Liaocheng, Shandong, China.
J Biochem Mol Toxicol. 2023 Dec;37(12):e23488. doi: 10.1002/jbt.23488. Epub 2023 Aug 19.
Colon cancer (CC) is a tumor of the large intestine. miR-92b-3p is often deregulated in the tumorigensis. Here, the role of miR-92b-3p in the development of CC was investigated. miR-92b-3p and Kruppel-like factor 3 (KLF3) expression was examined in CC tissues and cells. miR-92b-3p inhibitor or KLF3 overexpression vector was transfected into CC cells, respectively to observe its role in CC cell proliferation, invasion, migration, and apoptosis. The targeting relationship between miR-92b-3p and KLF3 was validated. Meanwhile, rescue experiments were performed by co-transfection of miR-92b-3p inhibitor and KLF3 siRNA, followed by determining CC cell proliferation, invasion, migration, and apoptosis. Higher miR-92b-3p and lower KLF3 expression levels were observed in CC tissues and cells. miR-92b-3p inhibition or KLF3 overexpression reduced proliferation, invasion, and migration whereas induced apoptosis of CC cells. KLF3 was validated to be the target gene of miR-92b-3p. Depletion of KLF3 could reverse the antitumor role of miR-92b-3p inhibition in CC cells. miR-92b-3p augments CC development through inhibiting KLF3, which may confers a novel way to develop future treatment target.
结肠癌（CC）是一种大肠肿瘤。miR-92b-3p在肿瘤发生过程中常常失调。在此，研究了miR-92b-3p在CC发生发展中的作用。检测了CC组织和细胞中miR-92b-3p和 Kruppel样因子3（KLF3）的表达。将miR-92b-3p抑制剂或KLF3过表达载体分别转染到CC细胞中，以观察其在CC细胞增殖、侵袭、迁移和凋亡中的作用。验证了miR-92b-3p与KLF3之间的靶向关系。同时，通过共转染miR-92b-3p抑制剂和KLF3 siRNA进行挽救实验，随后测定CC细胞的增殖、侵袭、迁移和凋亡。在CC组织和细胞中观察到较高的miR-92b-3p表达水平和较低的KLF3表达水平。抑制miR-92b-3p或过表达KLF3可降低CC细胞的增殖、侵袭和迁移，同时诱导其凋亡。验证KLF3是miR-92b-3p的靶基因。敲低KLF3可逆转miR-92b-3p抑制对CC细胞的抗肿瘤作用。miR-92b-3p通过抑制KLF3促进CC的发展这可能为未来开发治疗靶点提供一种新途径。
