Department of Forensic Sciences, The George Washington University, Washington, DC 20007, USA.
Department of Forensic Sciences, The George Washington University, Washington, DC 20007, USA.
J Chromatogr A. 2023 Sep 27;1707:464294. doi: 10.1016/j.chroma.2023.464294. Epub 2023 Aug 14.
For confirmation and/or screening purposes, rapid, selective, and precise chromatographic methods are required. In this vein, the utility of SiH columns (C18, UDC Cholesterol, and Diamond Hydride) with photodiode array UV absorption and single quadrupole MS detection for multi-modal separation of representative drugs from different drug classes on a single column using the same solvent reservoirs was investigated. For a conventional two column approach employing a combination of conventional C18 and silica columns operating in both the reversed phase chromatographic and hydrophilic interaction chromatographic modes, gradient analysis is required for the first column and there is a lack of retention on the second column for non-amine analytes. In comparison, all analytes are retained for two relatively rapid (< 10 min), precise (% RSD <0.4%), and non-correlated isocratic separations (R=0.2115) when using a UDC Cholesterol column.
为了确证和/或筛选目的，需要快速、选择性和精确的色谱方法。为此，我们研究了 SiH 柱（C18、UDC 胆固醇和 Diamond Hydride）与光电二极管阵列紫外吸收和单四极杆 MS 检测联用，在单根柱子上使用相同的溶剂储器，对来自不同药物类别的代表性药物进行多模式分离。对于传统的两柱方法，采用常规 C18 和硅胶柱的组合，分别在反相色谱和亲水相互作用色谱模式下运行，第一根柱子需要梯度分析，而对于非胺类分析物，第二根柱子没有保留。相比之下，使用 UDC 胆固醇柱时，所有分析物都能进行两次相对快速（<10 分钟）、精确（%RSD<0.4%）和非相关的等度分离（R=0.2115）。
