Research Center of Traditional Chinese Medicine Resources and Ethnic Medicine, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, 330004, PR China.
Department of Chemical and Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, 361021, Fujian province, PR China.
J Ethnopharmacol. 2024 Jan 10;318(Pt B):117055. doi: 10.1016/j.jep.2023.117055. Epub 2023 Aug 18.
Maxim is a traditional ethnic medicine commonly used in Tibet. In Tibetan medicine theory, Lagotis brachystachya is mainly used for the treatment of inflammatory related diseases. However, the active components and mechanism of the anti-inflammatory activity of Lagotis brachystachya are not clear.
The putative anti-inflammatory active compounds from Lagotis brachystachya Maxim and its anti-inflammation related mechanism involving in the TLR4/MyD88/NF-κB and NLRP3 signaling pathways were investigated.
In this study, we investigated the anti-inflammatory activity and mechanism of 32 compounds extracted from Lagotis brachystachya in HepG2 and THP-1 cells using the alcohol-induced HepG2 cell injury model and the monosodium urate (MSU) combined with lipopolysaccharide (LPS)-induced THP-1 cell inflammation model.
The results found that six compounds, including Echinacoside, Quercetin, Homoplantaginin, Tricin-7-O-glucoside, Apigenin and Luteolin-7-O-beta-d-glucopyranoside, were shown to exhibit significant anti-inflammatory effects in both cell models. Furthermore, these compounds were shown to inhibit the TLR4/MyD88/NF-κB and NLRP3 signaling pathways and reduce the release of pro-inflammatory cytokines IL-1β, TNF-α, and IL-6 in both cell models.
These findings suggest that Echinacoside, Quercetin, Homoplantaginin, Tricin-7-O-glucoside, Apigenin and Luteolin-7-O-beta-d-glucopyranoside from Lagotis brachystachya have promising potential as natural anti-inflammatory agents for the treatment of inflammatory-related diseases. The discovery of bioactive compounds from this plant opens up possibilities for the development of novel treatments for inflammatory-related diseases, potentially providing alternative or adjunctive options to conventional therapies.
川西小黄菊是藏医常用的传统民族药物。在藏医理论中，川西小黄菊主要用于治疗与炎症相关的疾病。然而，川西小黄菊抗炎活性的活性成分和作用机制尚不清楚。
本研究旨在从川西小黄菊中寻找具有抗炎活性的潜在化合物，并探讨其涉及 TLR4/MyD88/NF-κB 和 NLRP3 信号通路的抗炎相关机制。
本研究采用乙醇诱导的 HepG2 细胞损伤模型和尿酸单钠（MSU）联合脂多糖（LPS）诱导的 THP-1 细胞炎症模型，研究了从川西小黄菊中提取的 32 种化合物的抗炎活性和作用机制。
结果发现，包括 Echinacoside、Quercetin、Homoplantaginin、Tricin-7-O-glucoside、Apigenin 和 Luteolin-7-O-beta-d-glucopyranoside 在内的 6 种化合物在两种细胞模型中均表现出显著的抗炎作用。此外，这些化合物可抑制 TLR4/MyD88/NF-κB 和 NLRP3 信号通路，并降低两种细胞模型中促炎细胞因子 IL-1β、TNF-α 和 IL-6 的释放。
这些发现表明，川西小黄菊中的 Echinacoside、Quercetin、Homoplantaginin、Tricin-7-O-glucoside、Apigenin 和 Luteolin-7-O-beta-d-glucopyranoside 具有作为天然抗炎剂治疗炎症相关疾病的潜力。从该植物中发现生物活性化合物为炎症相关疾病的新型治疗方法的开发提供了可能性，可能为传统疗法提供替代或辅助选择。
