Hou Xuejuan, Nan Hui, Chen Xin, Ge Fei, Liu Yun, Li Feng, Zhang Dayi, Tian Jiang
Department of Environmental Science and Engineering, College of Environment and Resources, Xiangtan University, Xiangtan, China.
College of New Energy and Environment, Jilin University, Changchun, 130021, PR China; Key Laboratory of Groundwater Resources and Environment Ministry of Education, Jilin University, Changchun, 130021, PR China.
Environ Pollut. 2023 Nov 1;336:122408. doi: 10.1016/j.envpol.2023.122408. Epub 2023 Aug 17.
Glyphosate (Glp) was encapsulated onto the dopamine-modified attapulgite to develop an attapulgite-based nano-enabled Glp (DGlp) in this study with comparable weed control effects to pure Glp and commercial Glp solutions. Within 24 hours, the active Glp molecule was slowly released from DGlp at a maximum remaining rate of over 90%, and then degraded similarly to Glp solution in soil. The addition of DGlp improved soil available phosphorus (P) contents, phosphatase activity, and enzyme extractable P fraction. However, compared to Glp solution, DGlp addition had no effect on the transformation of soil inorganic P fractions. The 16S rRNA sequencing and co-occurrence network results revealed that DGlp had no significant effect on the soil bacterial diversity but diminished the complexity of soil bacterial network. According to the Mantel test, DGlp addition stimulated soil phosphatase activity and proliferation of dominant bacterial taxa (Proteobacteria and Firmicutes) capable of degrading Glp. Proteobacteria and Firmicutes that had been extensively recruited and enriched for their phosphatase activities may have mobilized reactive enzyme-P, significantly enhancing the transformation of reactive organic P and P-pool in soil. These results contributed to our understanding of the ecotoxicity and environmental impacts of nano-enabled Glp prior to its successful and sustainable application in agriculture.
在本研究中，草甘膦（Glp）被包裹在多巴胺修饰的凹凸棒石上，以开发一种基于凹凸棒石的纳米草甘膦（DGlp），其除草效果与纯草甘膦和市售草甘膦溶液相当。在24小时内，活性草甘膦分子从DGlp中缓慢释放，最大残留率超过90%，然后在土壤中与草甘膦溶液类似地降解。添加DGlp提高了土壤有效磷（P）含量、磷酸酶活性和酶可提取磷组分。然而，与草甘膦溶液相比，添加DGlp对土壤无机磷组分的转化没有影响。16S rRNA测序和共现网络结果表明，DGlp对土壤细菌多样性没有显著影响，但降低了土壤细菌网络的复杂性。根据Mantel检验，添加DGlp刺激了土壤磷酸酶活性以及能够降解草甘膦的优势细菌类群（变形菌门和厚壁菌门）的增殖。因磷酸酶活性而被大量招募和富集的变形菌门和厚壁菌门可能动员了活性酶磷，显著增强了土壤中活性有机磷和磷库的转化。这些结果有助于我们在纳米草甘膦成功且可持续地应用于农业之前，了解其生态毒性和环境影响。
