Mabuchi I, Nonomura Y
J Biochem. 1986 Jul;100(1):151-6. doi: 10.1093/oxfordjournals.jbchem.a121687.
Acrosomal actin bundles were isolated from the sperm of horseshoe crabs from four different sources, three from Asia and one from North America, and their protein constituents and structures were compared. The bundle from the American Limulus polyphemus sperm was composed of actin and two associated proteins of MW 95,000 and MW 52,000, as reported previously (Tilney, L. G. (1975) J. Cell Biol. 64, 289-310). However, those from the three Asian species (Tachypleus tridentatus, T. gigas, and Carcinoscorpius rotundicauda) were composed only of actin and the protein of MW 95,000. Electron microscopic and optical diffraction studies indicated that both the helical structures and the interfilament spacing of the actin filaments composing the acrosomal bundle were indistinguishable among the four species. These results suggest that the MW 95,000 protein crosslinks actin filaments in the bundle. Moreover, they support the idea that Limulus and the three Asian species have evolved independently from a common ancestor.
从四种不同来源的鲎精子中分离出顶体肌动蛋白束，其中三种来自亚洲，一种来自北美，并对它们的蛋白质成分和结构进行了比较。如先前报道（蒂尔尼，L.G.（1975年）《细胞生物学杂志》64卷，289 - 310页），来自美国大西洋鲎精子的肌动蛋白束由肌动蛋白以及分子量为95,000和52,000的两种相关蛋白组成。然而，来自三种亚洲鲎（中国鲎、巨鲎和圆尾鲎）的肌动蛋白束仅由肌动蛋白和分子量为95,000的蛋白质组成。电子显微镜和光学衍射研究表明，构成顶体束的肌动蛋白丝的螺旋结构和丝间间距在这四个物种中没有区别。这些结果表明，分子量为95,000的蛋白质使束中的肌动蛋白丝交联。此外，它们支持了大西洋鲎和三种亚洲鲎从共同祖先独立进化而来的观点。
