磷酸铝吸附破伤风-白喉疫苗和脂质体介导的破伤风-白喉疫苗的免疫原性特征
Immunogenic characterization of AlPO adsorbed Td vaccine and liposome-mediated Td vaccine.
作者信息
Shuhsadhe Remees, Vazhayil Junise, Ali Heyam Saad, Orsud Hiba, Mergani Ahmed Elmontaser Omer
机构信息
AL Shifa College of Pharmacy, Perinthalmanna, India.
Department of Pharmaceutics, AL Shifa College of Pharmacy, Perinthalmanna, India.
出版信息
Clin Exp Vaccine Res. 2023 Jul;12(3):232-239. doi: 10.7774/cevr.2023.12.3.232. Epub 2023 Jul 31.
PURPOSE
The purpose of this study was to compare the antigenic potency and stability of tetanus and diphtheria (Td) vaccines when combined with aluminum phosphate (AlPO) and liposome adjuvants.
MATERIALS AND METHODS
and analyses were conducted using the single radial immunodiffusion method and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The Td vaccines were prepared with AlPO adsorption and liposome-mediated delivery, and protein antigens were characterized using these methods.
RESULTS
The results revealed that the liposome-mediated Td vaccines exhibited higher immunogenicity compared to the AlPO-adsorbed Td vaccines. Additionally, the liposome-mediated Td vaccines demonstrated higher stability as native antigens.
CONCLUSION
This study highlights the importance of utilizing liposome adjuvants in vaccine development. The liposome-mediated Td vaccines showed enhanced immunogenicity and stability, making them a promising approach for improving vaccine efficacy. Understanding and optimizing adjuvant strategies can contribute to the development of effective vaccines against various diseases.
目的
本研究的目的是比较破伤风和白喉（Td）疫苗与磷酸铝（AlPO）和脂质体佐剂联合使用时的抗原效力和稳定性。
材料与方法
采用单向辐射免疫扩散法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分析。制备了采用AlPO吸附和脂质体介导递送的Td疫苗，并使用这些方法对蛋白质抗原进行表征。
结果
结果显示，与AlPO吸附的Td疫苗相比，脂质体介导的Td疫苗表现出更高的免疫原性。此外，脂质体介导的Td疫苗作为天然抗原表现出更高的稳定性。
结论
本研究突出了在疫苗开发中使用脂质体佐剂的重要性。脂质体介导的Td疫苗显示出增强的免疫原性和稳定性，使其成为提高疫苗效力的一种有前景的方法。理解和优化佐剂策略有助于开发针对各种疾病的有效疫苗。
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