Yun Yi, Yahui Jiang, Bobo Bai, Caifeng Zhang, Yanli Zhao
Medical College, Qinghai University, No. 29 Tongren Road, Xining, 810001, Qinghai, China.
Ocean University of China Infirmary, Qingdao, 266100, Shandong, China.
Heliyon. 2023 Jul 27;9(8):e18712. doi: 10.1016/j.heliyon.2023.e18712. eCollection 2023 Aug.
Chinese Tibetan medicine plays a crucial role in complementary anti-tumor treatments. This article aims to investigate the inhibitory effect of the alcoholic extract of Tibetan medicine Deziyangxin (DZYX) on the proliferation and migration of non-small cell lung cancer (NSCLC) cells, specifically LLC cells, as well as explore its potential mechanism of action.
The effect of the alcoholic extract on LLC cell viability was assessed using the CCK-8 method. The proliferation of LLC cells was evaluated using the EdU (5-Acetyl-2'-deoxyuridine) assay. Transwell assays were conducted to measure cell metastasis. Western blot analysis was performed to assess the expression of Cleaved Caspase-3, Bcl-2, Beciln-1, indicating the impact of DZYX on apoptosis and autophagy in LLC cells. Furthermore, the anti-tumor mechanism of DZYX was explored through transcriptome research and detection of Akt, p-Akt, p-mTOR protein levels.
The ethanol extract of DZYX exhibited a concentration-dependent and time-dependent inhibitory effect on LLC cell viability, with an IC50 of 406.1 μg/ml. Moreover, the ethanol extract of DZYX significantly reduced the migration ability of LLC cells. Additionally, the alcoholic extract of DZYX upregulated the expression of Cleaved Caspase-3 and Beciln-1 proteins, while downregulating the expression of Bcl-2 in LLC cells. Importantly, DZYX ethanol extract down regulated the expression of Akt and p-mTOR proteins in LLC cells, which combined with transcriptome results indicated that the drug exerted a multi-target and multi-pathway effect, primarily related to inhibiting the activation of the PI3K/AKT/m-TOR signaling pathway.
The alcoholic extract of DZYX demonstrates inhibitory effects on LLC cells, promoting apoptosis and autophagy. It is hypothesized that its anti-tumor mechanism is associated with the PI3K/AKT/m-TOR pathway.
中国藏药在辅助抗肿瘤治疗中发挥着关键作用。本文旨在研究藏药德子养心（DZYX）醇提物对非小细胞肺癌（NSCLC）细胞，特别是 LLC 细胞增殖和迁移的抑制作用，并探讨其潜在作用机制。
采用 CCK-8 法评估醇提物对 LLC 细胞活力的影响。使用 EdU（5-乙酰-2'-脱氧尿苷）检测法评估 LLC 细胞的增殖。进行 Transwell 检测以测量细胞转移。采用蛋白质免疫印迹分析评估 Cleaved Caspase-3、Bcl-2、Beciln-1 的表达，以表明 DZYX 对 LLC 细胞凋亡和自噬的影响。此外，通过转录组研究以及检测 Akt、p-Akt、p-mTOR 蛋白水平来探索 DZYX 的抗肿瘤机制。
DZYX 乙醇提取物对 LLC 细胞活力呈现浓度和时间依赖性抑制作用，IC50 为 406.1 μg/ml。此外，DZYX 乙醇提取物显著降低了 LLC 细胞的迁移能力。另外，DZYX 醇提物上调了 LLC 细胞中 Cleaved Caspase-3 和 Beciln-1 蛋白的表达，同时下调了 Bcl-2 的表达。重要的是，DZYX 乙醇提取物下调了 LLC 细胞中 Akt 和 p-mTOR 蛋白的表达，结合转录组结果表明该药物发挥了多靶点、多途径作用，主要与抑制 PI3K/AKT/m-TOR 信号通路的激活有关。
DZYX 醇提物对 LLC 细胞具有抑制作用，促进细胞凋亡和自噬。推测其抗肿瘤机制与 PI3K/AKT/m-TOR 通路有关。
