Massik Abdelhamid, Hibaoui Lahbib, Benboubker Moussa, Yahyaoui Ghita, Oumokhtar Bouchra, Mahmoud Mustapha
Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, MAR.
Human Pathology, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, MAR.
Cureus. 2023 Aug 17;15(8):e43629. doi: 10.7759/cureus.43629. eCollection 2023 Aug.
Carbapenem resistance in () is a public health problem worldwide. Although carbapenem resistance is emerging in Morocco, few studies have shown the epidemiological profile of carbapenemase genes in Moroccan healthcare facilities. The aim of this study was to characterize the molecular profile of the carbapenemase enzyme in from clinical isolates.
Clinical strains isolated in the laboratory from various samples were subjected to several phenotypic tests. Antibiotic susceptibility and identification were tested using Phoenix 100 (Becton Dickinson Co., Sparks, MD, USA) and Api 20 (bioMérieux, Marcy-l'Etoile, France). Simple phenotypic assays were used to detect carbapenemase oxacillinase (OXA) and metallo-β-lactamase (MBL) production, including the modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA) test. The detection of carbapenemase genes was performed by multiplex and simple polymerase chain reaction (PCR).
A total of 140 strains or 100% of isolates contained OXA-51 and ISbA1 sequences, 89% contained OXA-23 and OXA-58 sequences, and 1% contained OXA-24 sequence. The MBL genes were predominated by Verona integron-encoded metallo-β-lactamase (VIM) (56%), followed by Seoul imipenemase (SIM) (39%), German imipenemase (GIM) (37%), São Paulo metallo-β-lactamase (SPM) (13%), imipenemase (IMP) (11%), and New Delhi metallo-β-lactamase (NDM) (4%). Guyana extended-spectrum β-lactamase (GES) was not found in any isolation.
Our study shows a high frequency of carbapenem resistance in , as it reports a high molecular diversity of carbapenemase-encoding genes, mainly dominated by the carbapenemase ISaba1/OXA-23, which represents an emerging threat in our hospital.
[细菌名称]中的碳青霉烯耐药性是一个全球性的公共卫生问题。尽管摩洛哥也出现了碳青霉烯耐药性，但很少有研究表明摩洛哥医疗机构中碳青霉烯酶基因的流行病学特征。本研究的目的是对临床分离株中碳青霉烯酶的分子特征进行表征。
实验室从各种样本中分离出的临床菌株进行了多项表型试验。使用Phoenix 100（美国马里兰州斯帕克斯的贝克顿·迪金森公司）和Api 20（法国马西 - 埃图瓦勒的生物梅里埃公司）检测抗生素敏感性和进行鉴定。使用简单的表型试验来检测碳青霉烯酶、氧青霉烯酶（OXA）和金属β-内酰胺酶（MBL）的产生，包括改良Hodge试验（MHT）和乙二胺四乙酸（EDTA）试验。通过多重和简单聚合酶链反应（PCR）检测碳青霉烯酶基因。
总共140株菌株或100%的分离株含有OXA - 51和ISbA1序列，89%含有OXA - 23和OXA - 58序列，1%含有OXA - 24序列。MBL基因以维罗纳整合子编码的金属β-内酰胺酶（VIM）为主（56%），其次是首尔亚胺培南酶（SIM）（39%）、德国亚胺培南酶（GIM）（37%）、圣保罗金属β-内酰胺酶（SPM）（13%）、亚胺培南酶（IMP）（11%）和新德里金属β-内酰胺酶（NDM）（4%）。在任何分离株中均未发现圭亚那超广谱β-内酰胺酶（GES）。
我们的研究表明[细菌名称]中碳青霉烯耐药性的频率很高，因为它报告了碳青霉烯酶编码基因的高分子多样性，主要由碳青霉烯酶ISaba1/OXA - 23主导，这在我们医院是一种新出现的威胁。
