Noguchi Shunsuke, Matsui Asuka
Laboratory of Veterinary Radiation, Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan.
Laboratory of Veterinary Radiology, College of Life, Environment, and Advanced Sciences, Osaka Metropolitan University, Osaka, Japan.
Front Vet Sci. 2023 Aug 3;10:1239224. doi: 10.3389/fvets.2023.1239224. eCollection 2023.
Squamous cell carcinoma (SCC) occurring in the tonsils (TSCC) has a poorer prognosis than SCC occurring in other regions of the oral cavity (non-tonsillar SCC [NTSCC]) because it easily metastasizes to distant organs. This study aimed to elucidate the molecular mechanisms underlying the migration and invasion of TSCC cells .
This study focused on differential microRNA (miRNA) expression using microRNA microarrays and quantitative polymerase chain reaction in canine TSCC and NTSCC tissues and cell lines. A target gene of the miRNA involved in cell migration and invasion was validated by wound healing, transwell, and luciferase assays.
miR-203 expression was lower in TSCC tissues than in the normal oral mucosa and NTSCC tissues. Transfection of the miR-203 mimic resulted in the downregulation of mesenchymal marker protein expression and attenuation of cell migration and invasion in TSCC cells, but not in NTSCC cells. A dual-luciferase assay revealed that miR-203 directly targeted the mesenchymal transcription factor . SLUG overexpression enhances the migration of TSCC cells.
Our study indicates that the miR-203/SLUG axis may be involved in the metastatic mechanisms of TSCC.
扁桃体鳞状细胞癌（TSCC）比口腔其他部位发生的鳞状细胞癌（非扁桃体鳞状细胞癌[NTSCC]）预后更差，因为它容易转移至远处器官。本研究旨在阐明TSCC细胞迁移和侵袭的分子机制。
本研究利用微RNA微阵列和定量聚合酶链反应，聚焦于犬TSCC和NTSCC组织及细胞系中差异微RNA（miRNA）表达。通过伤口愈合、Transwell和荧光素酶测定法验证参与细胞迁移和侵袭的miRNA的靶基因。
TSCC组织中miR-203表达低于正常口腔黏膜和NTSCC组织。转染miR-203模拟物导致TSCC细胞中间质标记蛋白表达下调以及细胞迁移和侵袭减弱，但NTSCC细胞未出现此现象。双荧光素酶测定显示miR-203直接靶向间充质转录因子。SLUG过表达增强TSCC细胞的迁移。
我们的研究表明miR-203/SLUG轴可能参与TSCC的转移机制。
