Functional analysis of promoter variant and its possible implication in colorectal cancer immunotherapy.

BACKGROUND

Colorectal cancer (CRC) is a prevalent cancer, ranking as the third most common. Recent advances in our understanding of the molecular causes of this disease have highlighted the crucial role of tumor immune evasion in its initiation and progression. , a receptor that acts as a negative regulator of T cell responses, plays a pivotal role in this process, and genetic variations in have been linked to CRC susceptibility, prognosis, and response to therapy.

METHODS

We conducted a case-control study involving 98 CRC patients and 424 controls. We genotyped the c.-319C > T variant (rs5742909) and performed an association analysis by comparing allele frequencies between the patients and controls. To assess the potential functional impact of this variant, we first performed an analysis of transcription factor binding sites using Genomatix. Finally, to validate our findings, we conducted a luciferase reporter gene assay using different cell lines and an electrophoretic mobility shift assay (EMSA).

RESULTS

The case-control association analysis revealed a significant association between c.-319C > T and CRC susceptibility ( = 0.023; OR 1.89; 95% CI = 1.11-3.23). Genomatix analysis identified LEF1 and TCF7 transcription factors as specific binders to c.-319C. The reporter gene assay demonstrated notable differences in luciferase activity between the c.-319 C and T alleles in COS-7, HCT116, and Jurkat cell lines. EMSA analysis showed differences in TCF7 interaction with the C and T alleles.

CONCLUSION

c.-319C > T is associated with CRC susceptibility. Based on our functional validation results, we proposed that c.-319C > T alters gene expression at the transcriptional level, triggering a stronger negative regulation of T-cells and immune tumoral evasion.