Yan Xi-Zhong, Ma Li, Li Xiao-Fei, Chang Le, Liu Qing-Zhao, Song Cheng-Fei, Zhao Jin-Yu, Qie Xing-Tao, Deng Cai-Ping, Wang Chen-Zhu, Hao Chi
College of Plant Protection, Shanxi Agricultural University, Taigu, China.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Pest Manag Sci. 2023 Dec;79(12):5270-5282. doi: 10.1002/ps.7735. Epub 2023 Sep 7.
The diamondback moth, Plutella xylostella, has developed resistance to almost all insecticides used for its control. The 'push-pull' method has been shown as an effective control strategy to address this resistance challenge of P. xylostella. The key focus of the strategy is the identification of attractive or repellent volatile components. The aim of this study was to identify attractive volatile compounds released from host plants. Identified compounds were applied in the biological control of this pest.
Nine active compounds released into the headspace of seven cruciferous plant species were identified using gas chromatography-electroantennographic detection and gas chromatography-mass spectrometry. Electroantennographic detection-active compounds included five green leaf volatiles (hexanal, trans-2-hexen-1-ol, cis-3-hexen-1-ol, cis-3-hexenyl acetate, and 1-penten-3-ol), three isothiocyanates (isopropyl isothiocyanate, allyl isothiocyanate, and butyl isothiocyanate), and nonanal. Except for nonanal, all the identified green leaf volatiles and isothiocyanates elicited strong electrophysiological and behavioral responses in P. xylostella. The strongest attractive compounds, trans-2-hexen-1-ol and isopropyl isothiocyanate, were further evaluated in oviposition and field-trapping assays. Results showed that they both lured female moths to lay eggs, and were highly attractive to P. xylostella adults in field, especially when used in combination with yellow and green sticky boards. However, a blend of the two compounds showed no synergistic effect, but rather an antagonistic effect.
Green leaf volatiles and isothiocyanates were identified as key olfactory cues for host selection of P. xylostella. Trans-2- hexen-1-ol and isopropyl isothiocyanate were identified as candidate attractive compounds to serve in a 'push-pull' strategy for P. xylostella control. © 2023 Society of Chemical Industry.
小菜蛾已对几乎所有用于防治的杀虫剂产生抗性。“推-拉”法已被证明是应对小菜蛾这种抗性挑战的有效防治策略。该策略的关键重点是识别有吸引力或驱避性的挥发性成分。本研究的目的是识别寄主植物释放的有吸引力的挥发性化合物，并将鉴定出的化合物应用于该害虫的生物防治。
使用气相色谱-触角电位检测和气相色谱-质谱联用技术，鉴定出七种十字花科植物释放到顶空的九种活性化合物。触角电位检测活性化合物包括五种绿叶挥发物（己醛、反-2-己烯-1-醇、顺-3-己烯-1-醇、顺-3-己烯基乙酸酯和1-戊烯-3-醇）、三种异硫氰酸酯（异丙基异硫氰酸酯、烯丙基异硫氰酸酯和丁基异硫氰酸酯）以及壬醛。除壬醛外，所有鉴定出的绿叶挥发物和异硫氰酸酯在小菜蛾中均引发了强烈的电生理和行为反应。对最强的引诱化合物反-2-己烯-1-醇和异丙基异硫氰酸酯进行了产卵和田间诱捕试验的进一步评估。结果表明，它们都能引诱雌蛾产卵，并且在田间对小菜蛾成虫具有高度吸引力，尤其是与黄色和绿色粘虫板一起使用时。然而，这两种化合物的混合物没有协同作用，反而具有拮抗作用。
绿叶挥发物和异硫氰酸酯被确定为小菜蛾寄主选择的关键嗅觉线索。反-2-己烯-1-醇和异丙基异硫氰酸酯被确定为候选引诱化合物，可用于小菜蛾防治的“推-拉”策略。© 2023化学工业协会。
