Department of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing 100081, P.R. China.
Institute of Systems Biomedicine, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University Health Science Center, Beijing 100191, P.R. China.
Nanoscale. 2023 Sep 1;15(34):13971-13986. doi: 10.1039/d3nr02930a.
Oral diseases are usually caused by inflammation and bacterial infection. Reactive oxygen species (ROS), which come from both autologous inflammation tissue and bacterial infection, play an important role in this process. Thus, the elimination of excessive intracellular ROS can be a promising strategy for anti-inflammatory treatment. With the rapid development of nanomedicines, nanozymes, which can maintain the intracellular redox balance and protect cells against oxidative damage, have shown great application prospects in the treatment of inflammation-related diseases. However, their performance in pulpitis and their related mechanisms have yet to be explored. Herein, we prepared dozens of metallic nanoparticles with core-shell structures, and among them, chromium nanoparticles (NanoCr) were selected for their great therapeutic potential for pulpitis disease. NanoCr showed a broad antibacterial spectrum and strong anti-inflammatory function. Antibacterial assays showed that NanoCr could effectively inhibit a variety of common pathogens of oral infection. experiments offered evidence of the multienzyme activity of NanoCr and its function in suppressing ROS-induced inflammation reactions. The experimental results show that NanoCr has optimal antibacterial and anti-inflammatory properties in cell models, showing great potential for the treatment of pulpitis. Therefore, the use of NanoCr could become a new therapeutic strategy for clinical pulpitis.
口腔疾病通常由炎症和细菌感染引起。活性氧（ROS）来自自身炎症组织和细菌感染，在这个过程中起着重要作用。因此，消除过多的细胞内 ROS 可能是一种有前途的抗炎治疗策略。随着纳米医学的快速发展，纳米酶可以维持细胞内的氧化还原平衡并保护细胞免受氧化损伤，在治疗与炎症相关的疾病方面显示出巨大的应用前景。然而，它们在牙髓炎中的性能及其相关机制尚未得到探索。在此，我们制备了数十种具有核壳结构的金属纳米粒子，其中选择了具有巨大治疗潜力的铬纳米粒子（NanoCr）用于牙髓炎疾病的治疗。NanoCr 表现出广谱的抗菌谱和强大的抗炎功能。抗菌实验表明，NanoCr 可以有效抑制多种常见的口腔感染病原体。实验提供了 NanoCr 的多酶活性及其抑制 ROS 诱导的炎症反应的功能证据。实验结果表明，NanoCr 在细胞模型中具有最佳的抗菌和抗炎特性，在治疗牙髓炎方面具有巨大的潜力。因此，NanoCr 的使用可能成为临床牙髓炎的一种新的治疗策略。
