Akintayo Stephen Olusanmi, Hosseini Behnoush, Vahidinasab Maliheh, Messmer Marc, Pfannstiel Jens, Bertsche Ute, Hubel Philipp, Henkel Marius, Hausmann Rudolf, Voegele Ralf T, Lilge Lars
Department of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.
Department of Phytopathology, Institute of Phytomedicine, University of Hohenheim, Stuttgart, Germany.
Front Bioeng Biotechnol. 2023 Aug 7;11:1228386. doi: 10.3389/fbioe.2023.1228386. eCollection 2023.
strains are of interest in agricultural applications due to their beneficial interactions with plants, notable through their antimicrobial activity. The biocontrol ability of two new lipopeptides-producing strains ES1-02 and EFSO2-04, against fungal phytopathogens of spp., was evaluated and compared with reference strains QST713 and FZB42. All strains were found to be effective against the plant pathogens, with the new strains showing comparable antifungal activity to QST713 and slightly lower activity than FZB42. Lipopeptides and their isoforms were identified by high-performance thin-layer chromatography (HPTLC) and mass spectrometric measurements. The associated antifungal influences were determined in direct antagonistic dual culture assays, and the inhibitory growth effects on spp. as representatives of phytopathogenic fungi were determined. The effects on bacterial physiology of selected strains were analyzed by mass spectrometric proteomic analyses using nano-LC-MS/MS. Lipopeptide production analysis revealed that all strains produced surfactin, and one lipopeptide of the iturin family, including bacillomycin L by ES1-02 and EFSO2-04, while QST713 and FZB42 produced iturin A and bacillomycin D, respectively. Fengycin production was however only detected in the reference strains. As a result of co-incubation of strain ES1-02 with the antagonistic phytopathogen , an increase in surfactin production of up to 10-fold was observed, making stress induction due to competitors an attractive strategy for surfactin bioproduction. An associated global proteome analysis showed a more detailed overview about the adaptation and response mechanisms of , including an increased abundance of proteins associated with the biosynthesis of antimicrobial compounds. Furthermore, higher abundance was determined for proteins associated with oxidative, nitrosative, and general stress response. In contrast, proteins involved in phosphate uptake, amino acid transport, and translation were decreased in abundance. Altogether, this study provides new insights into the physiological adaptation of lipopeptide-producing strains, which show the potential for use as biocontrol agents with respect to phytopathogenic fungi.
由于其与植物的有益相互作用，特别是通过其抗菌活性，这些菌株在农业应用中备受关注。评估了两种新的产脂肽菌株ES1-02和EFSO2-04对植物病原菌的生物防治能力，并与参考菌株QST713和FZB42进行了比较。发现所有菌株对植物病原菌均有效，新菌株显示出与QST713相当的抗真菌活性，且活性略低于FZB42。通过高效薄层色谱（HPTLC）和质谱测量鉴定了脂肽及其异构体。在直接拮抗双重培养试验中确定了相关的抗真菌影响，并确定了对作为植物病原真菌代表的菌株的抑制生长作用。使用纳米液相色谱-质谱/质谱通过质谱蛋白质组分析来分析所选菌株对细菌生理学的影响。脂肽生产分析表明，所有菌株均产生表面活性素，以及iturin家族的一种脂肽，ES1-02和EFSO2-04产生芽孢杆菌霉素L，而QST713和FZB42分别产生iturin A和芽孢杆菌霉素D。然而，仅在参考菌株中检测到丰原素的产生。由于菌株ES1-02与拮抗植物病原菌共同孵育，观察到表面活性素产量增加高达10倍，这使得竞争导致的应激诱导成为表面活性素生物生产的一种有吸引力的策略。相关的全局蛋白质组分析显示了关于该菌株适应和反应机制的更详细概述，包括与抗菌化合物生物合成相关的蛋白质丰度增加。此外，确定与氧化应激、亚硝化应激和一般应激反应相关的蛋白质丰度更高。相比之下，参与磷酸盐摄取、氨基酸转运和翻译的蛋白质丰度降低。总之，本研究为产脂肽菌株的生理适应提供了新见解，这些菌株显示出作为植物病原真菌生物防治剂的潜力。
