Tani Y, Asahi S, Yamada H
J Nutr Sci Vitaminol (Tokyo). 1986 Apr;32(2):137-45. doi: 10.3177/jnsv.32.137.
Flavobacterium meningosepticum IFO 12535, a menaquinone (MK) producer, was mutagenized to improve productivity. A mutant, which was resistant to 1-hydroxy-2-naphthoate (HNA), was found to produce MK more abundantly: 34 mg/liter of culture broth and 5.5 mg/g of dry cells. The mutant was less sensitive to inhibition by HNA on MK biosynthesis than the wild-type strain. MK was isolated from cells of the mutant and identified as MK-6 based on its physicochemical characteristics. Mutants, which were given each KCN resistance, aromatic amino acid auxotrophy and no carotenoid productivity, did not show further increase of productivity.
脑膜败血黄杆菌IFO 12535是一种甲萘醌（MK）产生菌，为提高其产量进行了诱变处理。发现一株对1-羟基-2-萘甲酸（HNA）具有抗性的突变体能够更大量地产生MK：每升培养液中含34毫克，每克干细胞含5.5毫克。与野生型菌株相比，该突变体对HNA对MK生物合成的抑制作用不太敏感。从该突变体的细胞中分离出MK，并根据其理化特性鉴定为MK-6。赋予每种氰化钾抗性、芳香族氨基酸营养缺陷型且无类胡萝卜素产生能力的突变体，其产量并未进一步提高。
