1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Plac Starynkiewicza 1/3, 02-015 Warsaw, Poland.
Department of Methodology, Medical University of Warsaw, 02-097 Warsaw, Poland; Doctoral School, Medical University of Warsaw, 02-091 Warsaw, Poland.
Reprod Biol. 2023 Dec;23(4):100796. doi: 10.1016/j.repbio.2023.100796. Epub 2023 Aug 21.
This study aimed to assess the utility of circulating miR-125b-5p, miR-199a-3p, miR-451a, and miR-3613-5p as biomarkers of endometriosis.
Patients with stage III or IV of endometriosis according to the revised American Society of Reproductive Medicine (rASRM) staging classification, as well as control women, were recruited. We created a prospective study conducted on a group of 48 patients (n = 25 controls, n = 24 endometriosis) who had laparoscopic surgery. Blood samples were taken and plasma miRNA levels were measured by quantitative real-time polymerase chain reaction (RT-qPCR) and assessed with AUC and ROC curves.
MiR-451a and miR-3613-5p were significantly decreased in the plasma of endometriosis patients. miR-451a had a receiver-operating characteristic (ROC) area under the curve 0.8283 and miR-3613-5p had a ROC area under the curve 0.7617. The concentration of circulating miR-125b-5p and miR-199-3p did not differ between endometriosis patients and controls. Plasma miRNA levels did not change with BMI, smoking status, fertility problems, or menstrual pain according to the VAS scale (p > 0.05).
Circulating miR-451a and miR-3613-5p levels significantly differed between endometriosis and controls. However, the levels of miR-451a were discordant with previous studies. Therefore, miR-3613-5p may have better potential as the endometriosis biomarker. Circulating miR-125b-5p and miR-199a-3p cannot be used as reliable markers of endometriosis.
本研究旨在评估循环 miR-125b-5p、miR-199a-3p、miR-451a 和 miR-3613-5p 作为子宫内膜异位症生物标志物的效用。
根据修订后的美国生殖医学学会 (rASRM) 分期分类，招募了患有 III 期或 IV 期子宫内膜异位症的患者以及对照女性。我们创建了一项前瞻性研究，对一组 48 名接受腹腔镜手术的患者（n=25 名对照，n=24 名子宫内膜异位症）进行了研究。采集血样并通过实时定量聚合酶链反应 (RT-qPCR) 测量血浆 miRNA 水平，并通过 AUC 和 ROC 曲线进行评估。
子宫内膜异位症患者血浆中 miR-451a 和 miR-3613-5p 水平显著降低。miR-451a 的受试者工作特征 (ROC) 曲线下面积为 0.8283，miR-3613-5p 的 ROC 曲线下面积为 0.7617。子宫内膜异位症患者和对照组之间循环 miR-125b-5p 和 miR-199-3p 的浓度没有差异。根据 VAS 量表，血浆 miRNA 水平与 BMI、吸烟状况、生育问题或月经痛无关 (p>0.05)。
子宫内膜异位症患者和对照组之间循环 miR-451a 和 miR-3613-5p 水平显著不同。然而，miR-451a 的水平与之前的研究不一致。因此，miR-3613-5p 可能作为子宫内膜异位症生物标志物具有更好的潜力。循环 miR-125b-5p 和 miR-199a-3p 不能作为子宫内膜异位症的可靠标志物。
