Kanaujia R R, Miyamoto Y, Hatano E, Ikuta Y
J Reconstr Microsurg. 1986 Jul;2(4):213-20. doi: 10.1055/s-2007-1007025.
This study focuses on obtaining more information about the site of anastomosis, with three-dimensional examination using methylmethacrylate resin (Mercox) corrosion casts. Seventy microvascular anastomoses were performed in Wistar albino rats, divided into two groups before sacrifice. In Group 1 (35), Mercox was injected into the vessels, whereas in Group 2 (35), the tissues were prepared without injecting Mercox. Both groups were compared under the scanning electron microscope (SEM). The SEM preparation in Group 1 was simple, easy, and artifacts were significantly minimized. The anastomotic site could be well appreciated in three-dimensional views. Clear negative imprints of the endothelial surface were achieved without any breakage in the Mercox corrosion cast, and it can be reliably used in the evaluation of small vessel anastomosis.
本研究聚焦于通过使用甲基丙烯酸甲酯树脂(Mercox)腐蚀铸型进行三维检查,获取更多关于吻合部位的信息。在Wistar白化大鼠身上进行了70次微血管吻合,在处死前将其分为两组。第1组(35只),将Mercox注入血管,而第2组(35只),不注入Mercox制备组织。两组在扫描电子显微镜(SEM)下进行比较。第1组的SEM制备简单、容易,伪像显著减少。吻合部位在三维视图中能清晰显示。在Mercox腐蚀铸型中,内皮表面获得了清晰的负性印记,且无任何破损,它可可靠地用于评估小血管吻合。