School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan.
Clinical Pharmacy Department, Faculty of Pharmacy, Ain Shams University, 11566 Cairo, Egypt; Deaprtment of Obstertrics and Gynecology, University of Chicago, 60637 Chicago, IL, USA.
Biomed Pharmacother. 2023 Oct;166:115327. doi: 10.1016/j.biopha.2023.115327. Epub 2023 Aug 22.
Polycystic ovary syndrome (PCOS) is a complex endocrine disorder that affects women of reproductive age, characterized by androgen-induced oxidative stress leading to several metabolic disorders. In this study, we investigated the potential therapeutic effect of caffeic acid on PCOS and its underlying molecular mechanism. We used a human ovarian granulosa cell line (KGN cells) induced by hydrogen peroxide (HO) to examine how caffeic acid influences the protein expression of oxidative stress-induced apoptosis-related markers. Our results indicate that caffeic acid significantly inhibits intracellular reactive oxygen species (ROS) generation and safeguards KGN cells against oxidative stress. For the in vivo aspect of our study, female Sprague-Dawley (SD) rats were utilized to induce the PCOS model using dehydroepiandrosterone (DHEA). Caffeic acid was then administered to the rats for a duration of 6 weeks. The outcomes revealed that caffeic acid effectively improved irregular estrous cycles, fasting blood glucose levels, liver function, and lipid profiles in DHEA-induced PCOS rats. Additionally, it mitigated hyperandrogenism, enhanced steroidogenesis enzyme expression, and modulated apoptosis-related protein expression. Our findings strongly suggest that caffeic acid holds promising potential in reducing oxidative stress-induced damage and ameliorating PCOS-related complications by modulating ER stress.
多囊卵巢综合征（PCOS）是一种影响育龄妇女的复杂内分泌疾病，其特征是雄激素诱导的氧化应激导致多种代谢紊乱。在这项研究中，我们研究了咖啡酸对 PCOS 的潜在治疗作用及其潜在的分子机制。我们使用过氧化氢（HO）诱导的人卵巢颗粒细胞系（KGN 细胞）来研究咖啡酸如何影响氧化应激诱导的细胞凋亡相关标志物的蛋白表达。我们的结果表明，咖啡酸显著抑制细胞内活性氧（ROS）的产生，并保护 KGN 细胞免受氧化应激。在我们的研究的体内部分，我们使用脱氢表雄酮（DHEA）诱导雌性 Sprague-Dawley（SD）大鼠建立 PCOS 模型。然后，将咖啡酸给予大鼠 6 周。结果表明，咖啡酸可有效改善 DHEA 诱导的 PCOS 大鼠不规则发情周期、空腹血糖水平、肝功能和血脂谱。此外，它减轻了高雄激素血症，增强了甾体生成酶的表达，并调节了细胞凋亡相关蛋白的表达。我们的研究结果表明，咖啡酸通过调节内质网应激，具有减轻氧化应激诱导的损伤和改善 PCOS 相关并发症的潜力。
