Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Rua Botucatu, 740-2° andar-Vila Clementino, CEP:04023-062, Sao Paulo, Sao Paulo, Brasil.
Universidade Brasil, Sao Paulo, Sao Paulo, Brasil.
Aesthetic Plast Surg. 2024 Feb;48(3):510-518. doi: 10.1007/s00266-023-03549-y. Epub 2023 Aug 24.
Seroma is a frequent complication that can affect the final result of reconstructive and cosmetic surgeries.
This study evaluated the effectiveness of 5-Fluorouracil and 75% hypertonic glucose in preventing seroma in a mastectomy rat model, as well as cellular and vascular events in adjacent tissues. A left mastectomy with lymphadenectomy was performed in 60 Wistar-Albino female rats. Animals randomly allocated to the control group (Group I; n = 20) were sutured right after mastectomy. The intervention groups received 1.0 mL of 75% hypertonic glucose (Group II; n = 20) or 1.0 mL of 5-Fluorouracil (Group III; n = 20) at the surgical site before suturing. The assessment of the presence of seroma was performed in all animals at 24, 48, and 72 h and on the 7th and 12th postoperative day. After the 12th day, a tissue sample was taken from the surgical site and sent for histological analysis. The occurrence of seroma was assessed using GEE. A significance level of 5% was adopted.
Differences in seroma formation over time were observed for both Control Group I (p=0.041) and Intervention Group II (p<0.001). In Intervention Group III, there was no difference in the percentage and volume of seroma across the assessment points (p=0.627). When both the Control and Intervention Group II were compared to Intervention Group III, we found a reduction in seroma formation in the last group. The reduction in the inflammatory process was more regular to Intervention Group III.
In this animal model, 5-Fluorouracil was more effective in preventing seroma formation than 75% Hypertonic Glucose. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
血清肿是一种常见的并发症，可影响重建和美容手术的最终结果。
本研究评估了在大鼠乳房切除术模型中，5-氟尿嘧啶和 75%高渗葡萄糖预防血清肿的有效性，以及相邻组织中的细胞和血管事件。对 60 只 Wistar-Albino 雌性大鼠进行左侧乳房切除术和淋巴结切除术。动物随机分配到对照组（I 组；n=20），在乳房切除术后立即缝合。干预组在缝合前于手术部位分别接受 1.0 mL 75%高渗葡萄糖（II 组；n=20）或 1.0 mL 5-氟尿嘧啶（III 组；n=20）。所有动物在术后 24、48 和 72 小时以及第 7 和 12 天评估血清肿的存在情况。第 12 天后，从手术部位取组织样本进行组织学分析。使用广义估计方程评估血清肿的发生情况。采用 5%的显著性水平。
对照组 I（p=0.041）和干预组 II（p<0.001）的血清肿形成随时间的差异有统计学意义。干预组 III 各评估点的血清肿百分比和体积无差异（p=0.627）。当对照组 I 和干预组 II 与干预组 III 进行比较时，我们发现最后一组的血清肿形成减少。干预组 III 的炎症过程减少更有规律。
在这种动物模型中，5-氟尿嘧啶在预防血清肿形成方面比 75%高渗葡萄糖更有效。无等级分配 本刊要求作者对每一份符合循证医学分级标准的投稿进行分级。这排除了综述文章、书评和涉及基础科学、动物研究、尸体研究和实验研究的手稿。如需详细了解这些循证医学评级，请参阅目录或在线作者指南 www.springer.com/00266 。
