Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China.
Biosensors (Basel). 2023 Aug 11;13(8):808. doi: 10.3390/bios13080808.
Diuron (DU) abuse in weed removal and shipping pollution prevention always leads to pesticide residues and poses a risk to human health. In the current research, an innovative electrochemical sensor for DU detection was created using a glassy carbon electrode (GCE) that had been modified with chitosan-encapsulated multi-walled carbon nanotubes (MWCNTs-CS) combined with nitrogen-doped graphene quantum dots (NGQDs). The NGQDs were prepared by high-temperature pyrolysis, and the MWCNTs-CS@NGQDs composite was further prepared by ultrasonic assembly. TEM, UV-Vis, and zeta potential tests were performed to investigate the morphology and properties of MWCNTs-CS@NGQDs. CV and EIS measurements revealed that the assembly of MWCNTs and CS improved the electron transfer ability and effective active area of MWCNTs. Moreover, the introduction of NGQDs further enhanced the detection sensitivity of the designed sensor. The MWCNTs-CS@NGQDs/GCE electrochemical sensor exhibited a wide linear range (0.0812 μg mL), a low limit of detection (0.04 μg mL), and high sensitivity (31.62 μA (μg mL) cm) for DU detection. Furthermore, the sensor demonstrated good anti-interference performance, reproducibility, and stability. This approach has been effectively employed to determine DU in actual samples, with recovery ranges of 99.4104% in river water and 90.0~94.6% in soil. The developed electrochemical sensor is a useful tool to detect DU, which is expected to provide a convenient and easy analytical technique for the determination of various bioactive species.
敌草隆（DU）在除草和防止航运污染中的滥用，总是导致农药残留，并对人类健康构成威胁。在当前的研究中，使用经过壳聚糖包裹的多壁碳纳米管（MWCNTs-CS）与氮掺杂石墨烯量子点（NGQDs）结合修饰的玻碳电极（GCE），创建了一种用于 DU 检测的创新电化学传感器。通过高温热解制备 NGQDs，进一步通过超声组装制备 MWCNTs-CS@NGQDs 复合材料。通过 TEM、UV-Vis 和 zeta 电位测试研究了 MWCNTs-CS@NGQDs 的形貌和性质。CV 和 EIS 测量表明，MWCNTs 和 CS 的组装提高了 MWCNTs 的电子转移能力和有效活性面积。此外，NGQDs 的引入进一步提高了设计传感器的检测灵敏度。MWCNTs-CS@NGQDs/GCE 电化学传感器对 DU 的检测表现出宽线性范围（0.0812 μg mL）、低检测限（0.04 μg mL）和高灵敏度（31.62 μA（μg mL）cm）。此外，该传感器表现出良好的抗干扰性能、重现性和稳定性。该方法已成功应用于实际样品中 DU 的测定，在河水和土壤中的回收率范围分别为 99.4104%和 90.0~94.6%。开发的电化学传感器是一种用于检测 DU 的有用工具，有望为各种生物活性物质的测定提供一种方便易用的分析技术。
