Research Service, Birmingham VA Health Care System, Birmingham, AL 35233, USA.
Healthspan, Resilience & Performance, Florida Institute for Human and Machine Cognition, Pensacola, FL 32502, USA.
Int J Environ Res Public Health. 2023 Aug 17;20(16):6591. doi: 10.3390/ijerph20166591.
Traumatic spinal cord injury (SCI) results in wide-ranging cellular and systemic dysfunction in the acute and chronic time frames after the injury. Chronic SCI has well-described secondary medical consequences while acute SCI has unique metabolic challenges as a result of physical trauma, in-patient recovery and other post-operative outcomes. Here, we used high resolution mass spectrometry approaches to describe the circulating lipidomic and metabolomic signatures using blood serum from mice 7 d after a complete SCI. Additionally, we probed whether the aporphine alkaloid, boldine, was able to prevent SCI-induced changes observed using these 'omics platforms'. We found that SCI resulted in large-scale changes to the circulating lipidome but minimal changes in the metabolome, with boldine able to reverse or attenuate SCI-induced changes in the abundance of 50 lipids. Multiomic integration using xMWAS demonstrated unique network structures and community memberships across the groups.
创伤性脊髓损伤 (SCI) 导致损伤后急性和慢性时间范围内广泛的细胞和全身功能障碍。慢性 SCI 具有明确的继发性医疗后果，而急性 SCI 由于身体创伤、住院康复和其他术后结果而具有独特的代谢挑战。在这里，我们使用高分辨率质谱分析方法描述了 SCI 后 7 天小鼠血清中的循环脂质组学和代谢组学特征。此外，我们还研究了阿朴啡生物碱博来霉素是否能够预防这些“组学”平台观察到的 SCI 诱导的变化。我们发现，SCI 导致循环脂质组发生大规模变化，但代谢组变化很小，博来霉素能够逆转或减弱 SCI 诱导的 50 种脂质丰度的变化。使用 xMWAS 的多组学整合显示了不同组之间独特的网络结构和社区成员关系。
