Li Ying, Xu Yonghong, Wu Shunjiao, Wang Baohe, Li Yaying, Liu Yinghong, Wang Jia
Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing 400715, China.
College of Plant Protection, Southwest University, Chongqing 400715, China.
Insects. 2023 Jul 28;14(8):671. doi: 10.3390/insects14080671.
The oriental fruit fly, (Hendel), is a notorious invasive pest that has raised concerns worldwide. Validamycin has been demonstrated to be a very strong inhibitor against trehalase in a variety of organisms. However, whether validamycin can inhibit trehalase activity to suppress trehalose hydrolysis and affect any other relevant physiological pathways in remains unknown. In this study, the effects of validamycin injection on the synthesis and metabolism of trehalose and chitin were evaluated. The results show that validamycin injection significantly affected trehalase activity and caused trehalose accumulation. In addition, the downstream pathways of trehalose hydrolysis, including the synthesis and metabolism of chitin, were also remarkably affected as the expressions of the key genes in these pathways were significantly regulated and the chitin contents were changed accordingly. Intriguingly, the upstream trehalose synthesis was also affected by validamycin injection due to the variations in the expression levels of key genes, especially . Moreover, BdTPPC1 was predicted to have a binding affinity to validamycin, and the subsequent in vitro recombinant enzyme activity assay verified the inhibitory effect of validamycin on BdTPPC1 activity for the first time. These findings collectively indicate that validamycin can be considered as a promising potential insecticide for the management of .
东方果实蝇（Hendel）是一种臭名昭著的入侵害虫，已引起全球关注。井冈霉素已被证明是多种生物中海藻糖酶的一种非常强的抑制剂。然而，井冈霉素是否能抑制海藻糖酶活性以抑制海藻糖水解并影响东方果实蝇体内的任何其他相关生理途径仍不清楚。在本研究中，评估了注射井冈霉素对海藻糖和几丁质合成与代谢的影响。结果表明，注射井冈霉素显著影响海藻糖酶活性并导致海藻糖积累。此外，海藻糖水解的下游途径，包括几丁质的合成与代谢，也受到显著影响，因为这些途径中关键基因的表达受到显著调控，几丁质含量也相应改变。有趣的是，由于关键基因表达水平的变化，尤其是[具体基因名称未给出]，注射井冈霉素也影响了上游海藻糖合成。此外，预测BdTPPC1与井冈霉素具有结合亲和力，随后的体外重组酶活性测定首次证实了井冈霉素对BdTPPC1活性的抑制作用。这些发现共同表明，井冈霉素可被视为一种有前景的潜在杀虫剂，用于防治东方果实蝇。
