Andouard Deborah, Tilloy Valentin, Ribot Elodie, Mayeras Melissa, Diaz-Gonzalez Daniel, El Hamel Chahrazed, Piras-Douce Fabienne, Mantel Nathalie, Alain Sophie
Bacteriology-Virology-Hygien Department, National Reference Center for Herpesviruses, Centre Hospitalier Universitaire de Limoges, 87000 Limoges, France.
RESINFIT-Antimicrobials: Molecular Supports of Resistances and Therapeutic Innovations, UMR Inserm 1092, University of Limoges, 87000 Limoges, France.
Pathogens. 2023 Jul 27;12(8):985. doi: 10.3390/pathogens12080985.
Human cytomegalovirus (HCMV) is the leading cause of congenital viral infection, leading to a variety of symptoms in the unborn child that range from asymptomatic to death in utero. Our objective was to better understand the mechanisms of placental infection by HCMV clinical strains, particularly during the first trimester of pregnancy. We thus characterized and compared the replication kinetics of various HCMV clinical strains and laboratory strains by measuring viral loads in an ex vivo model of first trimester villi and decidua, and used NGS and PCA analysis to analyze the genes involved in cell tropism and virulence factors. We observed that first trimester villi and decidua are similarly permissive to laboratory and symptomatic strains, and that asymptomatic strains poorly replicate in decidua tissue. PCA analysis allowed us to segregate our clinical strains based on their clinical characteristics, suggesting a link between gene mutations and symptoms. All these results bring forth elements that can help better understand the mechanisms that induce the appearance of symptoms or in the congenitally infected newborn.
人巨细胞病毒（HCMV）是先天性病毒感染的主要原因，可导致未出生胎儿出现各种症状，从无症状到子宫内死亡。我们的目标是更好地了解HCMV临床菌株感染胎盘的机制，尤其是在妊娠早期。因此，我们通过测量妊娠早期绒毛和蜕膜的体外模型中的病毒载量，对各种HCMV临床菌株和实验室菌株的复制动力学进行了表征和比较，并使用NGS和PCA分析来分析参与细胞嗜性和毒力因子的基因。我们观察到，妊娠早期的绒毛和蜕膜对实验室菌株和有症状菌株的易感性相似，而无症状菌株在蜕膜组织中的复制较差。PCA分析使我们能够根据临床特征对临床菌株进行分类，这表明基因突变与症状之间存在联系。所有这些结果都提供了有助于更好地理解导致症状出现或先天性感染新生儿的机制的要素。
