Lira Ewerton C, Nascimento Antonio Rb, Bass Chris, Omoto Celso, Cônsoli Fernando L
Department of Entomology and Acarology, Luiz de Queiroz College of Agriculture (Esalq), University of São Paulo (USP), Piracicaba, São Paulo, Brazil.
Science and Engineering Research Support Facility (SERSF), University of Exeter, Cornwall, UK.
Pest Manag Sci. 2023 Dec;79(12):5349-5361. doi: 10.1002/ps.7745. Epub 2023 Sep 12.
Laboratory-selected resistant strains of Euschistus heros to thiamethoxam (NEO) and lambda-cyhalothrin (PYR) were recently reported in Brazil. However, the mechanisms conferring resistance to these insecticides in E. heros remain unresolved. We utilized comparative transcriptome profiling and single nucleotide polymorphism (SNP) calling of susceptible and resistant strains of E. heros to investigate the molecular mechanism(s) underlying resistance.
The E. heros transcriptome was assembled, generating 91 673 transcripts with a mean length of 720 bp and N50 of 1795 bp. Comparative gene expression analysis between the susceptible (SUS) and NEO strains identified 215 significantly differentially expressed (DE) transcripts. DE transcripts associated with the xenobiotic metabolism were all up-regulated in the NEO strain. The comparative analysis of the SUS and PYR strains identified 204 DE transcripts, including an esterase (esterase FE4), a glutathione-S-transferase, an ABC transporter (ABCC1) and aquaporins that were up-regulated in the PYR strain. We identified 9588 and 15 043 nonsynonymous SNPs in the PYR and NEO strains. One of the SNPs (D70N) detected in the NEO strain occurs in a subunit (α5) of the nAChRs, the target site of neonicotinoid insecticides. Nevertheless, this residue position in α5 is not conserved among insects.
Neonicotinoid and pyrethroid resistance in laboratory-selected E. heros is associated with a potential metabolic resistance mechanism by the overexpression of proteins commonly involved in the three phases of xenobiotic metabolism. Together these findings provide insight into the potential basis of resistance in E. heros and will inform the development and implementation of resistance management strategies against this important pest. © 2023 Society of Chemical Industry.
最近在巴西报道了实验室筛选出的对噻虫嗪（NEO）和高效氯氟氰菊酯（PYR）具有抗性的英雄猎蝽菌株。然而，英雄猎蝽对这些杀虫剂产生抗性的机制仍未明确。我们利用英雄猎蝽敏感和抗性菌株的比较转录组分析和单核苷酸多态性（SNP）检测来研究抗性背后的分子机制。
组装了英雄猎蝽的转录组，产生了91673个转录本，平均长度为720bp，N50为1795bp。敏感（SUS）菌株和NEO菌株之间的比较基因表达分析确定了215个显著差异表达（DE）的转录本。与异生物质代谢相关的DE转录本在NEO菌株中均上调。SUS菌株和PYR菌株的比较分析确定了204个DE转录本，包括一种酯酶（酯酶FE4）、一种谷胱甘肽-S-转移酶、一种ABC转运蛋白（ABCC1）和水通道蛋白，它们在PYR菌株中上调。我们在PYR和NEO菌株中分别鉴定出9588个和15043个非同义SNP。在NEO菌株中检测到的一个SNP（D70N）发生在新烟碱类杀虫剂的靶标位点——烟碱型乙酰胆碱受体（nAChRs）的一个亚基（α5）中。然而，α5中的这个残基位置在昆虫中并不保守。
实验室筛选的英雄猎蝽对新烟碱类和拟除虫菊酯类的抗性与异生物质代谢三个阶段中常见蛋白质的过表达所导致的潜在代谢抗性机制有关。这些发现共同为英雄猎蝽抗性的潜在基础提供了见解，并将为针对这种重要害虫的抗性管理策略的制定和实施提供参考。©2023化学工业协会。
