Centro de Café Alcides Carvalho, Instituto Agronômico, CEP 13020-902, Campinas, SP, Brazil.
Empresa de Pesquisa Agropecuária de Minas Gerais - Epamig, CEP 37200-000, Lavras, MG, Brazil.
Plant Dis. 2023 Dec;107(12):3810-3816. doi: 10.1094/PDIS-09-22-2282-RE. Epub 2023 Nov 28.
Developing coffee cultivars resistant to multiple diseases by combining resistance genes is a top priority in breeding programs. To create cultivars resistant to diseases and nematodes, we transferred genes for resistance to bacterial infections caused by pv. , which causes bacterial halo blight (BHB), and pv. , which causes bacterial leaf spots (BLS), into Arabica coffee. Genetic analyses were conducted on breeding populations to estimate the number and function of genes that confer resistance to BHB and BLS. In total, 2,109 plants in the F generation and reciprocal backcrosses were inoculated with pv. , while 1,996 plants were inoculated with pv. . Results showed that resistance to both pathogens had a heritability of 0.99, and the segregations of resistance indicated that each disease was controlled by a single dominant gene. The analyses also revealed that the resistance genes for BHB and BLS were linked, with an average distance of 10.75 cM between them on the same chromosome.
通过结合抗性基因来培育对多种疾病具有抗性的咖啡品种是育种计划的重中之重。为了培育对疾病和线虫具有抗性的品种，我们将对 pv. 引起的细菌性感染的抗性基因, , 和 pv. 引起的细菌性叶斑病 (BLS) 的抗性基因转入阿拉比卡咖啡。在育种群体中进行了遗传分析，以估计赋予对 BHB 和 BLS 抗性的基因的数量和功能。总共对 F 代和回交的 2,109 株植物进行了 pv. 的接种，而 1,996 株植物进行了 pv. 的接种。结果表明，两种病原体的抗性都具有 0.99 的遗传力，且抗性的分离表明每种疾病都由单个显性基因控制。分析还表明，BHB 和 BLS 的抗性基因是连锁的，它们在同一染色体上的平均距离为 10.75 cM。
