Verkhovskiĭ A B, Surgucheva I G, Gel'fand V I
Mol Biol (Mosk). 1986 Jul-Aug;20(4):922-8.
The method of isolation from bovine brain of a preparation containing 90 kDa- and 42 kDa-proteins is described. This preparation shortens actin filaments and therefore decreases viscosity of F-actin. The 42 kDa-component was identified as actin by one-dimensional peptide mapping. Quantitative densitometry has demonstrated that 90 kDa-protein and actin are present in the preparation in equimolar ratio. Fractionation of the preparation by gel-filtration, analytical centrifugation or electrophoresis under non-denaturing conditions showed that 90 kDa-protein and actin are in a light complex. This complex consists of one actin molecule and one molecule of 90 kDa-protein and has a sedimentation coefficient of 3.5S. Both beta- and gamma-isoelectric forms of actin are present in the complex.
描述了从牛脑中分离出含有90 kDa和42 kDa蛋白质制剂的方法。该制剂可缩短肌动蛋白丝，从而降低F-肌动蛋白的粘度。通过一维肽图分析，42 kDa成分被鉴定为肌动蛋白。定量光密度测定表明，制剂中90 kDa蛋白质和肌动蛋白以等摩尔比存在。在非变性条件下，通过凝胶过滤、分析离心或电泳对制剂进行分级分离，结果表明90 kDa蛋白质和肌动蛋白形成一个轻复合物。该复合物由一个肌动蛋白分子和一个90 kDa蛋白质分子组成，沉降系数为3.5S。复合物中同时存在β-和γ-等电形式的肌动蛋白。
