Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
Vertebrate Antibodies Ltd., Aberdeen AB24 2TZ, UK.
Cells. 2023 Aug 19;12(16):2097. doi: 10.3390/cells12162097.
Serum amyloid A (SAA) proteins belong to a family of acute-phase reactants, playing an integral role in defending the organism from pathological damage. Despite a wealth of data on the regulation of SAA transcripts in teleosts, there is only limited information on these proteins' abundance in fish. The aim of this study is to characterise SAA protein levels in salmonids using a newly developed antibody specific to salmonid SAA. The salmonid SAA antibody detected SAA and accurately discriminated between stimulated and control specimens from rainbow trout macrophage cell line (RTS-11) in vitro, as well as rainbow trout challenged with - or flagellin-stimulated Atlantic salmon in vivo. The presence of SAA protein was analysed in RTS-11 cell line supernatants, liver, and spleen samples using ELISA, immunoblotting, and immunohistochemistry. This study is the first to characterise SAA protein levels in salmonids in vivo and in vitro. The newly developed salmonid SAA antibody was able to discriminate between stimulated and unstimulated specimens, showing that it can be used to study the acute-phase response in salmonids with the potential to be further developed into assays to monitor and evaluate health in wild and farmed fish.
血清淀粉样蛋白 A（SAA）蛋白属于急性期反应物家族，在保护机体免受病理损伤方面发挥着重要作用。尽管有关硬骨鱼类 SAA 转录物调控的大量数据，但有关鱼类中这些蛋白质丰度的信息却非常有限。本研究旨在使用新开发的针对鲑鱼 SAA 的抗体来表征鲑鱼中的 SAA 蛋白水平。鲑鱼 SAA 抗体可检测 SAA，并可在体外的虹鳟巨噬细胞系（RTS-11）和体内受到刺激或鞭毛蛋白刺激的大西洋鲑中准确区分刺激和对照标本。使用 ELISA、免疫印迹和免疫组织化学分析 RTS-11 细胞系上清液、肝和脾样本中的 SAA 蛋白。本研究首次在体内和体外对鲑鱼中的 SAA 蛋白水平进行了表征。新开发的鲑鱼 SAA 抗体能够区分刺激和未刺激的标本，表明它可用于研究鲑鱼的急性期反应，并有潜力进一步开发成监测和评估野生和养殖鱼类健康的检测方法。
