Selection and Evaluation of Reference Genes for RT-qPCR Analysis in Konjac Based on Transcriptome Data.

Combined with the Konjac transcriptome database of our laboratory and internal reference genes commonly used in plants, the eight candidate internal reference genes were screened and detected. They are the 25S ribosomal RNA gene (), 18S ribosomal RNA gene (), actin gene (), glyceraldehyde-3-phosphate dehydrogenase gene (), ubiquitin gene (), β-tubulin gene (), eukaryotic elongation factor 1-αgene(), and eukaryotic translation initiation factor 4α-1 gene (). The results of GeNorm, Normfinder, and BestKeeper were analyzed comprehensively. The data showed that the expression levels of , , and at the reproductive periods, and at the nutritional periods, and , , and at different leaf developmental periods were stable. These identified and stable internal reference genes will provide the basis for the subsequent molecular biology-related studies of Konjac.