College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China.
Lotus Engineering Research Center of Hubei Province, College of Life Science, Wuhan University, Wuhan 430072, China.
Genes (Basel). 2023 Jul 25;14(8):1513. doi: 10.3390/genes14081513.
Combined with the Konjac transcriptome database of our laboratory and internal reference genes commonly used in plants, the eight candidate internal reference genes were screened and detected. They are the 25S ribosomal RNA gene (), 18S ribosomal RNA gene (), actin gene (), glyceraldehyde-3-phosphate dehydrogenase gene (), ubiquitin gene (), β-tubulin gene (), eukaryotic elongation factor 1-αgene(), and eukaryotic translation initiation factor 4α-1 gene (). The results of GeNorm, Normfinder, and BestKeeper were analyzed comprehensively. The data showed that the expression levels of , , and at the reproductive periods, and at the nutritional periods, and , , and at different leaf developmental periods were stable. These identified and stable internal reference genes will provide the basis for the subsequent molecular biology-related studies of Konjac.
结合本实验室的魔芋转录组数据库和植物中常用的内参基因，筛选和检测了 8 个候选内参基因。它们是 25S 核糖体 RNA 基因（）、18S 核糖体 RNA 基因（）、肌动蛋白基因（）、甘油醛-3-磷酸脱氢酶基因（）、泛素基因（）、β-微管蛋白基因（）、真核延伸因子 1-α 基因（）和真核翻译起始因子 4α-1 基因（）。综合分析了 GeNorm、Normfinder 和 BestKeeper 的结果。数据表明，在生殖期，在营养期，在不同叶片发育时期，和，和，的表达水平稳定。这些鉴定和稳定的内参基因将为魔芋后续的分子生物学相关研究提供基础。
