Alternative Splicing for Contributes to Variable Abiotic Stress Resistance in Transgenic Tobacco.

is a marsh plant in the family Compositae. It has good water and moisture resistance and ornamental properties, which makes it one of the important materials for chrysanthemum breeding and genetic improvement. The ( secondary wall enhancement factor 1) gene is associated with the thickening of the secondary walls of fiber cells in the plant ducts and the secondary xylem and plays an important role in plant stress resistance. In this study, two variable spliceosomes of the gene were identified from a chrysanthemum plant by using bioinformatics, qRT-PCR, transgene, and paraffin section methods to explore the molecular mechanism of the variable splicing of under abiotic stress. The results show that only three amino acids were found to be different between the two variants. After being treated with salt, drought, and low temperatures, analysis of the expression levels of the and genes in showed that could respond to low temperatures and salt stress and had a weak response to drought stress. However, the expression level of under the three treatments was lower than that of . transgenic tobacco showed increased saline-alkali resistance and low-temperature resistance at the seedling stage. transgenic tobacco also showed enhanced saline-alkali resistance and drought resistance at the seedling stage. In conclusion, the functions of the two variable spliceosomes of the gene are very different under abiotic stress. Therefore, this study verified the function of the variable spliceosome of and improved the stress resistance of the chrysanthemum plant under examination by regulating the expression of the protein, which lays a material foundation for the improvement of plant stress resistance materials and has important significance for the study of the resistance of chrysanthemum plants to abiotic stress.