Laboratory of Bacteriophage Biology, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, Prospect Nauki, 5, 142290 Pushchino, Russia.
Institute of Protein Research RAS, Institutskaya St., 4, 142290 Pushchino, Russia.
Int J Mol Sci. 2023 Aug 9;24(16):12584. doi: 10.3390/ijms241612584.
Bacteriophages are widely recognized as alternatives to traditional antibiotics commonly used in the treatment of bacterial infection diseases and in the food industry, as phages offer a potential solution in combating multidrug-resistant bacterial pathogens. In this study, we describe a novel bacteriophage, Kirov, which infects members of the group. Kirov is a broad-host-range phage belonging to the class. Its chromosome is a linear 165,667 bp double-stranded DNA molecule that contains two short, direct terminal repeats, each 284 bp long. According to bioinformatics predictions, the genomic DNA contains 275 protein-coding genes and 5 tRNA genes. A comparative genomic analysis suggests that Kirov is a novel species within the genus, belonging to the subfamily. Kirov demonstrates the ability to preserve and decontaminate from cow milk when present in milk at a concentration of 10 PFU/mL. After 4 h of incubation with the phage, the bacterial titer drops from 10 to less than 10 CFU/mL.
噬菌体被广泛认为是传统抗生素的替代品，常用于治疗细菌感染性疾病和食品工业，因为噬菌体在对抗多药耐药性细菌病原体方面提供了一种潜在的解决方案。在这项研究中，我们描述了一种新型噬菌体，Kirov，它感染 组的成员。Kirov 是一种广泛宿主范围的噬菌体，属于 类。它的染色体是一个线性的 165667 bp 双链 DNA 分子，包含两个短的、直接的末端重复序列，每个 284 bp 长。根据生物信息学预测，基因组 DNA 包含 275 个蛋白质编码基因和 5 个 tRNA 基因。比较基因组分析表明，Kirov 是 属中的一个新种，属于 亚科。Kirov 当以 10 PFU/mL 的浓度存在于牛奶中时，能够保持和清除牛奶中的 。在用噬菌体孵育 4 小时后，细菌滴度从 10 降至低于 10 CFU/mL。
