Capper S J, Venn R F, Morley J S
Neuropeptides. 1986 Jul;8(1):33-44. doi: 10.1016/0143-4179(86)90062-4.
Highly specific antisera have been raised to [Leu]enkephalin and applied in a radioimmunoassay that is sufficiently sensitive and precise for the measurement of enkephalin pentapeptides in body fluids. Extraction of CSF by Sep-pak cartridges, followed by reverse-phase HPLC to separate [Leu] and [Met]enkephalin prior to assay, has assessed their concentrations as 59-170 pmol/l and 0.5-30 pmol/l, respectively, in three patients with chronic pain. The reproducibility of the HPLC separation step was checked by adding to the sample a synthetic analogue of very low cross-reactivity in UV detectable quantities. The assay procedure described is generally applicable to the assessment of [Leu] and [Met]enkephalin concentrations in CSF.
已制备出针对亮氨酸脑啡肽的高特异性抗血清，并将其应用于一种放射免疫测定法中，该方法对于测量体液中的脑啡肽五肽具有足够的灵敏度和精密度。通过Sep-pak柱提取脑脊液，然后在测定前用反相高效液相色谱法分离亮氨酸脑啡肽和甲硫氨酸脑啡肽，在三名慢性疼痛患者中，分别测定其浓度为59 - 170 pmol/L和0.5 - 30 pmol/L。通过向样品中添加紫外可检测量的低交叉反应性合成类似物来检查高效液相色谱分离步骤的重现性。所描述的测定方法一般适用于评估脑脊液中亮氨酸脑啡肽和甲硫氨酸脑啡肽的浓度。
