Division of Rheumatology, Allergy and Immunology, New Taipei Municipal Tucheng Hospital, New Taipei City 236, Taiwan.
Division of Rheumatology, Allergy and Immunology, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, Taiwan.
Int J Mol Sci. 2023 Aug 20;24(16):12998. doi: 10.3390/ijms241612998.
The enzymes α-2,6-sialyltransferase 1 (ST6Gal1), neuraminidase 1 (Neu1), α-2,3-sialyltransferase 1 (ST3Gal1), and neuraminidase 3 (Neu3) are known to affect immune cell function. However, it is not known whether the levels of these enzymes relate to remission definitions or differentiate American College of Rheumatology (ACR), European League Against Rheumatism (EULAR), and Simplified Disease Activity Index (SDAI) responses in patients with rheumatoid arthritis (RA). We measured the ST6Gal1, Neu1, ST3Gal1, and Neu3 levels of B cells and monocytes in RA patients and correlated the cells' enzyme levels/ratios with the improvement in the ACR, EULAR and SDAI responses and with the two remission definitions. The difference in the B-cell Neu1 levels differed between the ACR 70% improvement and non-improvement groups ( = 0.043), between the EULAR good major response (improvement) and non-good response groups ( = 0.014), and also between the SDAI 50% or 70% improvement and non-improvement groups ( = 0.001 and 0.018, respectively). The same held true when the RA patients were classified by positive rheumatoid factor or the use of biologics. The B-cell Neu1 levels significantly indicated 2005 modified American Rheumatism Association and 2011 ACR/EULAR remission definitions (area under the curve (AUC) = 0.674 with = 0.001, and AUC = 0.682 with < 0.001, respectively) in contrast to the CRP and ESR (all AUCs < 0.420). We suggest that B-cell Neu1 is superior for discriminating ACR, EULAR, and SDAI improvement and is good for predicting two kinds of remission definitions.
α-2,6-唾液酸转移酶 1（ST6Gal1）、神经氨酸酶 1（Neu1）、α-2,3-唾液酸转移酶 1（ST3Gal1）和神经氨酸酶 3（Neu3）等酶已知会影响免疫细胞功能。然而，目前尚不清楚这些酶的水平是否与缓解定义有关，或者是否可以区分美国风湿病学会（ACR）、欧洲抗风湿病联盟（EULAR）和简化疾病活动指数（SDAI）在类风湿关节炎（RA）患者中的反应。我们测量了 RA 患者 B 细胞和单核细胞中的 ST6Gal1、Neu1、ST3Gal1 和 Neu3 水平，并将细胞的酶水平/比值与 ACR、EULAR 和 SDAI 反应的改善以及两种缓解定义相关联。B 细胞 Neu1 水平在 ACR70%改善和未改善组之间存在差异（=0.043），在 EULAR 良好主要反应（改善）和非良好反应组之间存在差异（=0.014），并且在 SDAI50%或 70%改善和未改善组之间存在差异（分别为=0.001 和 0.018）。当 RA 患者按阳性类风湿因子或生物制剂的使用进行分类时，情况也是如此。B 细胞 Neu1 水平显著表明 2005 年美国风湿病学会修订标准和 2011 年 ACR/EULAR 缓解定义（曲线下面积（AUC）=0.674，P=0.001，AUC=0.682，P<0.001），而 C 反应蛋白和红细胞沉降率（所有 AUC<0.420）则不然。我们建议 B 细胞 Neu1 更适合区分 ACR、EULAR 和 SDAI 的改善，并且对于预测两种缓解定义也很有价值。
