Lukáčová Alexandra, Lihanová Diana, Beck Terézia, Alberty Roman, Vešelényiová Dominika, Krajčovič Juraj, Vesteg Matej
Department of Biology, Ecology and Environment, Faculty of Natural Sciences, Matej Bel University, 974 01 Banská Bystrica, Slovakia.
Institute of Biology and Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, 917 01 Trnava, Slovakia.
Life (Basel). 2023 Aug 12;13(8):1734. doi: 10.3390/life13081734.
Phenol, a monocyclic aromatic hydrocarbon with various commercial uses, is a major pollutant in industrial wastewater. is a unicellular freshwater flagellate possessing secondary chloroplasts of green algal origin. This protist has been widely used for monitoring the biological effect of various inorganic and organic environmental pollutants, including aromatic hydrocarbons. In this study, we evaluate the influence of different phenol concentrations (3.39 mM, 3.81 mM, 4.23 mM, 4.65 mM, 5.07 mM, 5.49 mM and 5.91 mM) on the growth, morphology and cell division of . The cell count continually decreases ( < 0.05-0.001) over time with increasing phenol concentration. While phenol treatment does not induce bleaching (permanent loss of photosynthesis), the morphological changes caused by phenol include the formation of spherical ( < 0.01-0.001), hypertrophied ( < 0.05) and monster cells ( < 0.01) and lipofuscin bodies. Phenol also induces an atypical form of cell division of , simultaneously producing more than 2 (3-12) viable cells from a single cell. Such atypically dividing cells have a symmetric "star"-like shape. The percentage of atypically dividing cells increases ( < 0.05) with increasing phenol concentration. Our findings suggest that can be used as bioindicator of phenol contamination in freshwater habitats and wastewater.
苯酚是一种具有多种商业用途的单环芳烃，是工业废水中的主要污染物。[某种生物名称]是一种具有绿藻起源的次生叶绿体的单细胞淡水鞭毛虫。这种原生生物已被广泛用于监测各种无机和有机环境污染物（包括芳烃）的生物效应。在本研究中，我们评估了不同苯酚浓度（3.39 mM、3.81 mM、4.23 mM、4.65 mM、5.07 mM、5.49 mM和5.91 mM）对[某种生物名称]的生长、形态和细胞分裂的影响。随着苯酚浓度的增加，细胞计数随时间持续下降（<0.05 - 0.001）。虽然苯酚处理不会导致漂白（光合作用的永久丧失），但苯酚引起的形态变化包括球形细胞的形成（<0.01 - 0.001）、肥大细胞（<0.05）和畸形细胞（<0.01）以及脂褐素小体。苯酚还诱导[某种生物名称]出现非典型的细胞分裂形式，从单个细胞同时产生超过2个（3 - 12个）活细胞。这种非典型分裂的细胞具有对称的“星”状形状。非典型分裂细胞的百分比随着苯酚浓度的增加而增加（<0.05）。我们的研究结果表明，[某种生物名称]可作为淡水生境和废水中苯酚污染的生物指示物。
