Ramírez-Noguera Patricia, Zetina Marín Iliane, Gómez Chavarin Blanca Margarita, Valderrama Moisés Eduardo, López-Barrera Laura Denise, Díaz-Torres Roberto
Multidisciplinary Research Unit, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan Km. 2.5, San Sebastián Xhala, Cuautitlán Izcalli CP 54714, Mexico.
School of Medicine, Universidad Nacional Autónoma de México, Circuito Interior, Ciudad Universitaria, Av. Universidad 3000, Mexico City CP 04510, Mexico.
Pharmaceutics. 2023 Aug 21;15(8):2172. doi: 10.3390/pharmaceutics15082172.
Due to cartilage's limited capacity for regeneration, numerous studies have been conducted to find new drugs that modify osteoarthrosis's progression. Some evidence showed the capability of chitosan nanoparticles with glutathione (Np-GSH) to regulate the oxide-redox status in vitro in human chondrocytes. This work aimed to evaluate the capacity of Np-GSH in vivo, using Wistar rats with induced surgical osteoarthritis. Radiographic, biochemical (GSH and TBARS quantification), histopathological, and immunohistochemical (Col-2 and MMP-13) analyses were performed to evaluate the progress of the osteoarthritic lesions after the administration of a single dose of Np-GSH. According to the results obtained, the GSH contained in the NPs could be vectored to chondrocytes and used by the cell to modulate the oxidative state reduction, decreasing the production of ROS and free radicals induced by agents oxidizing xenobiotics, increasing GSH levels, as well as the activity of GPx, and decreasing lipid peroxidation. These results are significant since the synthesis of GSH develops exclusively in the cell cytoplasm, and its quantity under an oxidation-reduction imbalance may be defective. Therefore, the results allow us to consider these nanostructures as a helpful study tool to reduce the damage associated with oxidative stress in various diseases such as osteoarthritis.
由于软骨的再生能力有限，人们进行了大量研究以寻找能改变骨关节炎进展的新药。一些证据表明，壳聚糖纳米颗粒与谷胱甘肽（Np-GSH）在体外对人软骨细胞具有调节氧化还原状态的能力。本研究旨在利用诱导手术性骨关节炎的Wistar大鼠评估Np-GSH在体内的作用。在单次给予Np-GSH后，进行了放射学、生化分析（谷胱甘肽和硫代巴比妥酸反应物定量）、组织病理学和免疫组织化学分析（Ⅱ型胶原和基质金属蛋白酶-13），以评估骨关节炎病变的进展。根据所得结果，纳米颗粒中所含的谷胱甘肽可被输送至软骨细胞，并被细胞用于调节氧化态的降低，减少由异源生物氧化剂诱导产生的活性氧和自由基，提高谷胱甘肽水平以及谷胱甘肽过氧化物酶的活性，并减少脂质过氧化。这些结果意义重大，因为谷胱甘肽的合成仅在细胞质中进行，在氧化还原失衡情况下其数量可能存在缺陷。因此，这些结果使我们能够将这些纳米结构视为一种有用的研究工具，以减少与骨关节炎等各种疾病中氧化应激相关的损伤。
