一种由杂交骨髓瘤单克隆抗体所定义的人类胸腺细胞抗原。

A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.

作者信息

McMichael A J, Pilch J R, Galfré G, Mason D Y, Fabre J W, Milstein C

出版信息

Eur J Immunol. 1979 Mar;9(3):205-10. doi: 10.1002/eji.1830090307.

Abstract

Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of HTA 1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 molecular weight.

摘要

用人类胸腺细胞免疫的BALB/c小鼠的脾细胞与骨髓瘤细胞系P3-NS 1/1 Ag 4.1进行融合。产生的一个杂交克隆（NA 1/34）分泌出一种对人类胸腺细胞具有高度特异性的抗体。85%的胸腺细胞表达名为HTA1的抗原。每个细胞估计有15×10⁴个HTA 1分子，因此它是一种主要的表面分子。如另一种单克隆抗体W6/32所识别的，这种抗原在胸腺细胞上的表达似乎与HLA呈相反关系。对[¹²⁵I]标记的胸腺细胞膜的免疫沉淀物质进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析，结果显示有一个分子量为45,000的单一成分。