Norwegian Institute for Food, Fisheries and Aquaculture Research, Muninbakken 9-13, Breivika, Tromsø, 9291, Norway; Faculty of Science and Technology, Norwegian University of Life Sciences, Ås, 1432, Norway.
Norwegian Institute for Food, Fisheries and Aquaculture Research, Muninbakken 9-13, Breivika, Tromsø, 9291, Norway.
Talanta. 2024 Jan 1;266(Pt 2):125079. doi: 10.1016/j.talanta.2023.125079. Epub 2023 Aug 19.
In this work, we evaluated the feasibility of Raman spectroscopy as an in-line raw material characterization tool for industrial process control of the hydrolysis of poultry rest raw material. We established calibrations (N = 59) for fat, protein, ash (proxy for bone) and hydroxyproline (proxy for collagen) in ground poultry rest raw material. Calibrations were established in the laboratory using poultry samples with high compositional variation. Samples were measured using a wide area illumination Raman probe at varying working distance (6 cm, 9 cm, 12 cm) and probe tilt angle (0°, 30°) to mimic expected in-line variations in the measurement situation. These moderate variations did not significantly affect performance for any analytes. The obtained calibrations were tested in-line with continuous measurements of the ground poultry by-product stream at a commercial hydrolysis facility over the course of two days. Measurements were acquired under demanding conditions, e.g. large variations in working distance. Reasonable estimates of compositional trends were obtained. Validation samples (N = 19) were also reasonably well predicted, with RMSEP = [0.14, 1.37, 2.36, 1.51]% for hydroxyproline, protein, fat and ash, respectively. However, there were indications that further calibration development and robustification of pre-processing would be advantageous, particularly with respect to hydroxyproline and protein models. It is the authors' impression that with such efforts, potentially in combination with development of practical measurement setup, the use of Raman spectroscopy as a process control tool for the hydrolysis of poultry rest raw materials is within reach.
在这项工作中，我们评估了拉曼光谱作为一种在线原材料特性工具，用于工业过程控制水解家禽残余原料的可行性。我们为地面家禽残余原料中的脂肪、蛋白质、灰分（代表骨骼）和羟脯氨酸（代表胶原蛋白）建立了校准（N=59）。校准工作是在实验室中使用具有高成分变化的家禽样本进行的。使用宽面积照明拉曼探头，在不同的工作距离（6cm、9cm、12cm）和探头倾斜角度（0°、30°）下测量样本，以模拟预期的在线测量情况中的变化。这些适度的变化对任何分析物的性能都没有显著影响。获得的校准在商业水解设施中连续测量地面家禽副产品流的两天内进行了在线测试。在苛刻的条件下进行了测量，例如工作距离的较大变化。获得了合理的成分趋势估计。还使用验证样本（N=19）对获得的校准进行了测试，羟脯氨酸、蛋白质、脂肪和灰分的 RMSEP 值分别为[0.14、1.37、2.36、1.51]%。然而，有迹象表明，进一步的校准开发和预处理稳健化将是有利的，特别是对于羟脯氨酸和蛋白质模型。作者的印象是，通过这些努力，可能结合实际测量设置的开发，拉曼光谱作为水解家禽残余原料的过程控制工具的使用是可行的。
