Shenzhen Engineering Research Laboratory for Sludge and Food Waste Treatment and Resource Recovery, Tsinghua Shenzhen International Graduate School, Tsinghua University, China; School of Environmental Science and Engineering, Sun Yat-sen University, China.
Shenzhen Engineering Research Laboratory for Sludge and Food Waste Treatment and Resource Recovery, Tsinghua Shenzhen International Graduate School, Tsinghua University, China; Shenzhen Environmental Science and New Energy Laboratory, Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, China.
Water Res. 2023 Oct 1;244:120512. doi: 10.1016/j.watres.2023.120512. Epub 2023 Aug 19.
The microbial community of a sequencing batch reactor operated under feast and famine conditions for production of polyhydroxyalkanoates (PHAs) was characterized through high-throughput sequencing and metagenomic analysis. The fermented food waste and chemically-enhanced primary sludge was fed in this bioreactor. After acclimation, the PHA yield achieved as high as 0.60-0.69 g COD/g COD. The complete changes of microbial community structure were found during shifts of feedstock. A synthesis of SCL/MCL-PHAs pathway was established for PHA-producing bioreactor in this mixed-culture system. The structure-performance relationship of PHA-producing microbial community and feedstock composition was investigated. The results showed that microbial community tends to be decentralized and prefer team work for PHA synthesis to consume the multiple substrates and digest inevitable non-VFA contents in fermented liquor. This study also discovered unreported potential PHA producers (e.g., genera Tabrizicola, Nannocystis, Ga0077539, Ga0077559, JOSHI-001, SNC69-320 and UBA2334) subsisting on municipal organic wastes and expands the current knowledge about mixed-culture system that the PHA synthesis pathway is widely existed in activated sludge.
在生产聚羟基烷酸酯 (PHA) 的序批式反应器中，通过高通量测序和宏基因组分析研究了在丰食和饥饿条件下运行的微生物群落。该生物反应器中使用发酵的食物垃圾和化学增强的初沉污泥作为进料。经过驯化后，PHA 得率高达 0.60-0.69 g COD/g COD。在进料变化过程中发现了微生物群落结构的完全变化。在混合培养系统中，为 PHA 生产生物反应器建立了 SCL/MCL-PHA 合成途径。研究了 PHA 生产微生物群落与进料组成的结构-性能关系。结果表明，微生物群落倾向于分散化，并倾向于团队合作进行 PHA 合成，以消耗多种底物并消化发酵液中不可避免的非 VFA 含量。本研究还发现了未报道的潜在 PHA 生产者（例如，Tabrizicola、Nannocystis、Ga0077539、Ga0077559、JOSHI-001、SNC69-320 和 UBA2334 属），它们以城市有机废物为食，并扩展了关于混合培养系统的现有知识，即 PHA 合成途径广泛存在于活性污泥中。
