Institute of Traditional Chinese Veterinary Medicine & MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China.
Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agriculture and Animal Husbandry Science, Lhasa, 850009, China.
Microb Pathog. 2023 Oct;183:106322. doi: 10.1016/j.micpath.2023.106322. Epub 2023 Aug 24.
During the last decade, researchers had started to focus on the relationship between intestinal parasitic infection and variation of intestinal microflora. Cryptosporidium is a widely known opportunistic and zoonotic pathogen. Several studies have shown that Cryptosporidium infection has impact to alter the gut microflora. However, there are only few studies referring to the fungal microflora changes in response to Cryptosporidium infection in highland ruminants. Therefore, the present study was performed for exploring the alternations of intestinal fungal microbiota in yaks after exposure to Cryptosporidium infection. In present study, Amplicon sequencing of ITS regions was used to study the variations of fungal microflora in yaks. After filtering the raw data, over 45 000 and 62 000 clean data were obtained in uninfected and infected yaks, respectively. By using alpha diversity analysis, it was found that there is no significant difference in the richness and evenness when positive samples were compared with negative ones, whereas intestinal fungal communities in different taxa in yaks were changed. The results of present study depicted that 2-phyla and 21-genera in the infected animals had significantly (P < 0.05) changed. These genera were Septoria, Coniothyrium, Cleistothelebolus, Bensingtonia, Cystobasidium, Filobasidium, Coprotus, Carex, Blumeria, Coprinellus, Leucosporidium, Phialophora, Isolepis, Ascobolus, Thecaphora, Mortierella, Urocystis, Symmetrospora and Lasiobolus. In addition, we found variations in 28 enzymes suggesting that the function of microbiota was also affected. It is concluded that there are drastic changes in the fungal microflora and microbiota functions after exposure to Cryptosporidium infection in yak. Our results help to focus on the prompt way for the development of new therapies to control Cryptosporidiosis.
在过去的十年中，研究人员开始关注肠道寄生虫感染与肠道微生物群变异之间的关系。隐孢子虫是一种广泛存在的机会性和动物源性病原体。多项研究表明，隐孢子虫感染会影响肠道微生物群的改变。然而，关于高山反刍动物感染隐孢子虫后真菌微生物群变化的研究很少。因此，本研究旨在探索感染隐孢子虫后牦牛肠道真菌微生物群的变化。本研究采用 ITS 区扩增子测序方法研究牦牛肠道真菌微生物群的变化。对原始数据进行过滤后，分别获得了未感染和感染牦牛的 45000 多个和 62000 多个清洁数据。通过 alpha 多样性分析，发现阳性样本与阴性样本相比，丰富度和均匀度没有显著差异，但牦牛肠道不同分类群的真菌群落发生了变化。本研究结果表明，感染动物的 2 个门和 21 个属发生了显著变化（P < 0.05）。这些属包括Septoria、Coniothyrium、Cleistothelebolus、Bensingtonia、Cystobasidium、Filobasidium、Coprotus、Carex、Blumeria、Coprinellus、Leucosporidium、Phialophora、Isolepis、Ascobolus、Thecaphora、Mortierella、Urocystis、Symmetrospora 和 Lasiobolus。此外，我们还发现 28 种酶的变化，表明微生物群的功能也受到了影响。综上所述，牦牛感染隐孢子虫后，真菌微生物群和微生物群功能发生了剧烈变化。我们的研究结果有助于关注开发新疗法控制隐孢子虫病的即时途径。
