Environmental Biology Research Laboratory, Department of Botany, Utkal University, Bhubaneswar, Odisha, India.
Environmental Biology Research Laboratory, Department of Botany, Utkal University, Bhubaneswar, Odisha, India; Center of Environment, Climate Change and Public Health, Utkal University, Vani Vihar, Bhubaneswar 751004, Odisha, India.
Int J Biol Macromol. 2023 Dec 1;252:126503. doi: 10.1016/j.ijbiomac.2023.126503. Epub 2023 Aug 24.
Cyanobacterial polyhydroxybutyrate (PHB) is preferred over bacteria for low-cost production due to its photoautotrophic nature and lower carbon requirement. Considering its impact on the environment and circular economy, the valorization of fruit waste is the need of the hour. In the present study, fruit peels of banana, orange, pea, jackfruit, watermelon and waste flowers were tried as carbon sources for mangrove-isolated cyanobacteria Limnothrix planktonica to accumulate PHB. Alterations in the ASN-III culture medium and the introduction of untreated and pre-treated (acid/alkali-treated) peels as carbon sources are tried to enhance PHB. Banana peel showed the maximum PHB accumulation potential of 25.73 mg/L on the 12th day of incubation, followed by jackfruit (22.46 mg/L) and watermelon peels (20.72 mg/L); whereas, commercial carbon sources showed lower PHB accumulation up to 19.26 mg/L and 18.21 mg/L with fructose and glucose respectively. PHB accumulation was boosted to 5-fold higher (39.39 mg/L) in NP deficiency medium along with banana peel supplement, as compared to photoautotrophic conditions (8.49 mg/L) after the 9th day of incubation. Additionally, the PHB obtained by using the fruit wastes has a higher molecular weight than the PHB accumulated during photoautotrophic conditions. Optimization of parameters using fruit wastes and characterization of PHB would lead to its potential use.
与细菌相比，由于其自养特性和较低的碳需求，蓝细菌聚羟基丁酸酯（PHB）更适合低成本生产。考虑到其对环境和循环经济的影响，水果废物的增值利用是当前的需要。在本研究中，尝试将香蕉、橙子、豌豆、菠萝蜜、西瓜和废花的果皮作为红树林分离的浮游蓝细菌 Limnothrix planktonica 积累 PHB 的碳源。尝试改变 ASN-III 培养基，并引入未经处理和预处理（酸/碱处理）的果皮作为碳源，以提高 PHB 的产量。香蕉皮在培养的第 12 天显示出最大的 PHB 积累潜力，为 25.73 mg/L，其次是菠萝蜜（22.46 mg/L）和西瓜皮（20.72 mg/L）；而商业碳源的 PHB 积累量分别为 19.26 mg/L 和 18.21 mg/L，果糖和葡萄糖的含量较低。与光自养条件下（第 9 天培养时为 8.49 mg/L）相比，在 NP 缺乏培养基中加入香蕉皮补充物后，PHB 积累量增加了 5 倍（39.39 mg/L）。此外，与光自养条件下积累的 PHB 相比，使用水果废物获得的 PHB 具有更高的分子量。通过使用水果废物优化参数并对 PHB 进行表征，将有助于其潜在应用。
