门腔分流大鼠中促胰液素刺激的心脏腺苷酸环化酶活性的改变。

Alteration of secretin-stimulated cardiac adenylate cyclase activity in rats with portacaval shunt.

作者信息

De Fontaine S, Robberecht P, De Neef P, Christophe J

出版信息

Peptides. 1986 Jul-Aug;7(4):645-9. doi: 10.1016/0196-9781(86)90040-9.

Abstract

Wistar rats were submitted to portacaval anastomosis (PCA). Control rats were sham-operated and pair-fed (SOPF). After 3 weeks, PCA led to the hypertrophy of right atrium (+50%), left atrium (+67%) and both ventricles (+26%). The response of adenylate cyclase activity to secretin was specifically and markedly decreased in membranes from atria (-51 to 59%) and ventricles (-68 to 69%). These data suggest a decrease in the number of functional secretin receptors in heart considering that: the half-maximal stimulatory secretin concentration was unchanged; glucagon stimulations were unaltered and D,L-isoproterenol stimulations were hardly affected; the Gpp(NH)p-, NaF-, and forskolin-stimulated adenylate cyclase activities were moderately decreased (in ventricles, by 14-28%) or unchanged (in atria).

摘要

将Wistar大鼠进行门腔静脉吻合术（PCA）。对照组大鼠进行假手术并配对喂养（SOPF）。3周后，PCA导致右心房（+50%）、左心房（+67%）和两个心室（+26%）肥大。心房（-51%至59%）和心室（-68%至69%）膜中腺苷酸环化酶活性对促胰液素的反应特异性且显著降低。这些数据表明心脏中功能性促胰液素受体数量减少，原因如下：促胰液素的半数最大刺激浓度未改变；胰高血糖素刺激未改变，D,L-异丙肾上腺素刺激几乎未受影响；Gpp(NH)p、NaF和福斯高林刺激的腺苷酸环化酶活性中度降低（心室中降低14%至28%）或未改变（心房中）。