Wang Ming-Cheng, Fan Yu-Hua, Zhang Yen-Zhen, Bregente Carl Jay Ballena, Lin Wei-Hung, Chen Chao-An, Lin Tzu-Ping, Kao Cheng-Yen
Division of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Taiwan.
Department of Urology, Taipei Veterans General Hospital, Taipei, Taiwan; Department of Urology, College of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Infect Genet Evol. 2023 Oct;114:105493. doi: 10.1016/j.meegid.2023.105493. Epub 2023 Aug 25.
In this study, we compared the characteristics of different uropathogenic Escherichia coli phylogroups. A total of 844 E. coli isolated from urine were enrolled and the antimicrobial susceptibility of E. coli to 22 antibiotics was determined by disk diffusion test. The distribution of phylogroups and 20 virulence factor genes was determined by PCR. Phenotypes associated with bacterial virulence, including motility, biofilm formation, and the production of curli and siderophore, were examined. Phylogroup B2 was dominant in our isolates (64.8%), followed by phylogroups D (8.6%), B1 (7.8%), F (6.0%), C (4.5%), A (3.1%), untypable (2.8%), E (1.8%), and clade I (0.5%). The prevalence of multidrug-resistant strains was highest in phylogroup C (86.8%), followed by E (80.0%), F (75.0%), and D (71.2%). Moreover, 23.5% of the phylogroup F E. coli were extensively drug-resistant. Phylogroup B2 E. coli had an average of the highest virulence factor genes (10.1 genes/isolate). Compared to phylogroup B2 E. coli, phylogroups F and clade I E. coli had higher motility while phylogroup C E. coli had lower motility. >60% of phylogroups A and C E. coli showed very low curli production. In contrast, 14%, 10%, and 7%, of E. coli in phylogroups F, B2, and E, produced a very high amount of curli, respectively. Surprisingly, phylogroup A E. coli showed the highest virulence to larvae, followed by phylogroups B2 and C. In summary, we first characterized and revealed that the antimicrobial resistance, virulence gene distribution, motility, and curli production, were associated with in E. coli phylogroups.
在本研究中，我们比较了不同尿路致病性大肠杆菌菌群的特征。共纳入了844株从尿液中分离出的大肠杆菌，通过纸片扩散试验测定了大肠杆菌对22种抗生素的药敏性。通过聚合酶链反应（PCR）确定了菌群和20种毒力因子基因的分布。检测了与细菌毒力相关的表型，包括运动性、生物膜形成以及卷曲菌毛和铁载体的产生。在我们分离出的菌株中，B2菌群占主导地位（64.8%），其次是D菌群（8.6%）、B1菌群（7.8%）、F菌群（6.0%）、C菌群（4.5%）、A菌群（3.1%）、无法分型的菌群（2.8%）、E菌群（1.8%）和进化枝I（0.5%）。多重耐药菌株的流行率在C菌群中最高（86.8%），其次是E菌群（80.0%）、F菌群（75.0%）和D菌群（71.2%）。此外，F菌群的大肠杆菌中有23.5%为广泛耐药。B2菌群的大肠杆菌平均具有最高的毒力因子基因数量（10.1个基因/菌株）。与B2菌群的大肠杆菌相比，F菌群和进化枝I的大肠杆菌具有更高的运动性，而C菌群的大肠杆菌运动性较低。超过60%的A菌群和C菌群的大肠杆菌卷曲菌毛产生量极低。相比之下，F菌群、B2菌群和E菌群的大肠杆菌分别有14%、10%和7%产生大量卷曲菌毛。令人惊讶的是，A菌群的大肠杆菌对幼虫表现出最高的毒力，其次是B2菌群和C菌群。总之，我们首次对大肠杆菌菌群的抗菌耐药性、毒力基因分布、运动性和卷曲菌毛产生进行了表征并揭示了它们之间的关联。
