Liang Yunlai, Ma Yating, Wang Kun, Xiang Manglin, Yi Bin
Department of Clinical Laboratory, Xiangya Hospital of Central South University, No. 87, Xiangya Street, Kaifu District, Changsha, 410008, Hunan, China.
Cancer Cell Int. 2023 Aug 27;23(1):181. doi: 10.1186/s12935-023-03038-x.
The association of NUCB-2/Nesfatin-1 with nasopharyngeal carcinoma (NPC) remains unclear. We clarified the role of NUCB-2/Nesfatin-1 in the development, progression and diagnosis of NPC.
In nasopharyngeal carcinoma cell lines (5-8 F, 6-10B, CNE1, CNE2 and NP69), western blotting, MTT, EdU and other techniques were performed to investigate the role of NUCB-2 in nasopharyngeal carcinoma. 70 tissue samples (39 NPC and 31 rhinitis) and 140 serum samples (including NPC, rhinitis, other head and neck tumors and healthy control) were included to explore the expression of NUCB-2 and its metabolite Nesfatin-1 in tissues or serum of patients with nasopharyngeal carcinoma.
NUCB-2 level in NPC tissue was higher than that in rhinitis tissue (P < 0.05). Suppression of NUCB-2 in the NPC cell line CNE2 inhibited proliferation and clone formation of the cells; on the contrary, improvement of NUCB-2 in the NPC cell line CNE1 promoted cell propagation and clone development. An elevated serum level of NUCB-2 in NPC patients was detected, compared to that in patients with other head and neck tumors, rhinitis or healthy donors. Determination of nesfatin-1 combined with EA-IgA, VCA-IgA and Rta-IgG in serum samples for NPC diagnosis reached a sensitivity of 93.6% and a specificity of 94.5%, while the positive and negative predictive value of this diagnostic model was 89.8% and 96.6%, and the accuracy yielded 94.2%.
This study revealed that NUCB-2 could enhance proliferation of NPC cells and NUCB-2/nesfatin-1 has the potential to be a serological marker to aid early diagnosis of nasopharyngeal carcinoma.
NUCB - 2/nesfatin - 1与鼻咽癌（NPC）之间的关联尚不清楚。我们阐明了NUCB - 2/nesfatin - 1在鼻咽癌发生、发展及诊断中的作用。
在鼻咽癌细胞系（5 - 8F、6 - 10B、CNE1、CNE2和NP69）中，采用蛋白质免疫印迹法、MTT法、EdU法等技术研究NUCB - 2在鼻咽癌中的作用。纳入70份组织样本（39份鼻咽癌组织和31份鼻炎组织）以及140份血清样本（包括鼻咽癌、鼻炎、其他头颈部肿瘤及健康对照），以探究NUCB - 2及其代谢产物nesfatin - 1在鼻咽癌患者组织或血清中的表达情况。
鼻咽癌组织中NUCB - 2水平高于鼻炎组织（P < 0.05）。抑制鼻咽癌CNE2细胞系中的NUCB - 2可抑制细胞增殖和克隆形成；相反，提高鼻咽癌CNE1细胞系中的NUCB - 2则促进细胞增殖和克隆发展。检测发现，鼻咽癌患者血清中NUCB - 2水平高于其他头颈部肿瘤患者、鼻炎患者或健康供体。血清样本中nesfatin - 1联合EA - IgA、VCA - IgA和Rta - IgG用于鼻咽癌诊断时，灵敏度达93.6%，特异度达94.5%，该诊断模型的阳性预测值和阴性预测值分别为89.8%和96.6%，准确率为94.2%。
本研究表明，NUCB - 2可增强鼻咽癌细胞的增殖能力，且NUCB - 2/nesfatin - 1有潜力成为辅助鼻咽癌早期诊断的血清学标志物。
