Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH - Royal Institute of Technology, Stockholm, Sweden.
Methods Mol Biol. 2024;2713:519-541. doi: 10.1007/978-1-0716-3437-0_34.
Cell morphology and motility drive the cellular capabilities to interact with the environment. For example, microglia, the longest known tissue-resident macrophages, show a highly branched process tree with which they continuously scan their environment. Computational image analysis allows to quantify morphology and/or motility from images of tissue-resident macrophages. Here, I describe a step-by-step protocol for analyzing the morphology (and motility) of macrophages with our recently described, freely available software MotiQ, which provides a broad band of parameters and thereby serves as a versatile tool for studies of morphology and motility.
细胞形态和运动能力决定了细胞与环境相互作用的能力。例如，小胶质细胞是已知的最长的组织驻留巨噬细胞，它们表现出高度分支的过程树，用它来不断扫描它们的环境。计算图像分析允许从组织驻留巨噬细胞的图像中定量形态和/或运动。在这里，我描述了一个使用我们最近描述的免费软件 MotiQ 分析巨噬细胞形态（和运动）的分步协议，该软件提供了广泛的参数，因此是研究形态和运动的通用工具。
