College of Basic Medicine, Beihua University, Jilin, PR China.
College of Basic Medicine, Beihua University, Jilin, PR China.
Gene. 2023 Oct 30;884:147744. doi: 10.1016/j.gene.2023.147744. Epub 2023 Aug 26.
This paper explored using of deer antlers as a model for studying rapid growth and cartilage formation in mammals. The genes and regulatory mechanisms involved in antler chondrogenesis are poorly understood, however, previous research has suggested that DNA methylation played a key role in antler regeneration. By using fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP), this study measured DNA methylation levels in cartilage (CA) and reserve mesenchyme (RM) cells and tissues. Results showed that RM cells (RMCs) DNA methylation levels were significantly lower than those of CA, suggesting that DNA demethylation may be involved in antler fast cartilage differentiation. The study also identified 20 methylated fragments specific to RMCs or CA using the methylation-sensitive amplified polymorphism (MSAP) technique and confirmed these findings using southern blot analysis. The data provide the first experimental evidence of a link between epigenetic regulation and rapid cartilage differentiation in antlers.
本文探讨了利用鹿角作为研究哺乳动物快速生长和软骨形成的模型。然而，鹿角软骨发生过程中涉及的基因和调控机制仍知之甚少，先前的研究表明，DNA 甲基化在鹿角再生中起着关键作用。本研究使用荧光标记的甲基化敏感扩增多态性（F-MSAP）技术，测量了软骨（CA）和储备间充质（RM）细胞和组织中的 DNA 甲基化水平。结果表明，RM 细胞（RMCs）的 DNA 甲基化水平明显低于 CA，提示 DNA 去甲基化可能参与了鹿角快速软骨分化。该研究还使用甲基化敏感扩增多态性（MSAP）技术鉴定了 20 个特异于 RMC 或 CA 的甲基化片段，并通过 Southern blot 分析验证了这些发现。该数据首次提供了实验证据，表明表观遗传调控与鹿角中快速软骨分化之间存在联系。
