Marichal Laurent, Bagnard Lucie, Sire Olivier, Vendrely Charlotte, Bruckert Franz, Weidenhaupt Marianne
Université Grenoble Alpes, CNRS, Grenoble-INP LMGP, Grenoble F-38000, France.
IRDL, UMR CNRS 6027, Université Bretagne Sud, Vannes, France.
Biochim Biophys Acta Gen Subj. 2023 Nov;1867(11):130450. doi: 10.1016/j.bbagen.2023.130450. Epub 2023 Aug 26.
Functional amyloids are commonly produced by many microorganisms and their biological functions are numerous. Staphylococcus aureus can secrete a group of peptides named phenol-soluble modulins (PSMs) in their biofilm extracellular matrix. PSMs have been found inside biofilms both in their soluble form and assembled into amyloid structures. Yet, the actual biological function of these amyloids has been highly debated. Here, we assessed the ability of PSMs to form amyloids in contact with different abiotic surfaces to unravel a potential unknown bioadhesive and/or biofilm stabilization function. We combined surface plasmon resonance imaging, fluorescence aggregation kinetics, and FTIR spectroscopy in order to evaluate the PSM adsorption as well as amyloid formation properties in the presence of various surface chemistries. Overall, PSMs adsorb even on low-binding surfaces, making them highly adaptable adsorbants in the context of bioadhesion. Moreover, the PSM aggregation potential to form amyloid aggregates is not impacted by the presence of the surface chemistries tested. This versatility regarding adsorption and amyloid formation may imply a possible role of PSMs in biofilm adhesion and/or structure integrity.
功能性淀粉样蛋白通常由许多微生物产生，其生物学功能众多。金黄色葡萄球菌可在其生物膜细胞外基质中分泌一组名为酚溶性调节蛋白（PSMs）的肽。已在生物膜中发现PSMs以可溶性形式存在，并组装成淀粉样结构。然而，这些淀粉样蛋白的实际生物学功能一直存在激烈争论。在此，我们评估了PSMs与不同非生物表面接触时形成淀粉样蛋白的能力，以揭示潜在的未知生物粘附和/或生物膜稳定功能。我们结合了表面等离子体共振成像、荧光聚集动力学和傅里叶变换红外光谱，以评估在各种表面化学条件下PSMs的吸附以及淀粉样蛋白形成特性。总体而言，PSMs即使在低结合表面上也能吸附，使其在生物粘附方面成为高度适应性的吸附剂。此外，形成淀粉样聚集体的PSM聚集潜力不受所测试表面化学物质存在的影响。这种在吸附和淀粉样蛋白形成方面的多功能性可能意味着PSMs在生物膜粘附和/或结构完整性中可能发挥作用。
