Larsen T H, Saetersdal T, Grong K
Res Exp Med (Berl). 1986;186(4):295-306. doi: 10.1007/BF01852306.
The ultrastructure of myocytes was studied in the left ventricular myocardium of the cat heart after 3 h of LAD ligation. The ischemic, borderline, and normally perfused myocardium was defined by in vivo injection of fluorescein and by regional myocardial blood flow measurements with 15.5 microns radio-labeled microspheres. A semiquantitation of the number of irreversible injured cells in per cent of total counted in the three different zones showed that 53%-63% were irreversibly injured in the ischemic zone, 7%-26% in the borderline area, while none were irreversibly injured in the normally perfused myocardium. The interior excitation-contraction couplings in the normally perfused myocardium comprise interior dyads, triads, reversed triads, and encircling couplings. While the couplings in general were structurally resistant to ischemia, injured interior couplings were apparent in severely damaged cells of the hypoperfused tissue. Such injuries comprise a widening of the junctional gap and a disintegration of the junctional processes.
在结扎左冠状动脉前降支3小时后，对猫心脏左心室心肌细胞的超微结构进行了研究。通过活体注射荧光素以及使用15.5微米放射性标记微球测量局部心肌血流量，来界定缺血、边缘和正常灌注的心肌。对三个不同区域中不可逆损伤细胞数量占总计数的百分比进行半定量分析，结果显示，缺血区有53%-63%的细胞发生不可逆损伤，边缘区为7%-26%，而正常灌注心肌中无细胞发生不可逆损伤。正常灌注心肌中的内部兴奋-收缩偶联包括内部二联体、三联体、反向三联体和环绕偶联。虽然这些偶联总体上在结构上对缺血有抵抗力，但在灌注不足组织的严重受损细胞中，可见受损的内部偶联。此类损伤包括连接间隙增宽和连接突起解体。
